Topoisomerase II is a structural component of mitotic chromosome scaffolds

William C. Earnshaw, Brian Halligan, Carol A. Cooke, Margarete M S Heck, Leroy-Fong Liu

Research output: Contribution to journalArticle

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Abstract

We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase II, an abundant nuclear enzyme that controls DNA topological states: anti-scaffold antibody inhibits the strand-passing activity of DNA topoisomerase II; and both anti-scaffold antibody and an independent antibody raised against purified bovine topoisomerase II recognize identical partial proteolysis fragments of the 170,000-mol-wt scaffold protein in immunoblots. Our results suggest that topoisomerase II may be an enzyme that is also a structural protein of interphase nuclei and mitotic chromosomes.

Original languageEnglish
Pages (from-to)1706-1715
Number of pages10
JournalJournal of Cell Biology
Volume100
Issue number5
DOIs
Publication statusPublished - May 1 1985
Externally publishedYes

Fingerprint

Type II DNA Topoisomerase
Chromosomes
Interphase
Anti-Idiotypic Antibodies
Peptides
Proteins
Antibodies
Enzymes
Indirect Fluorescent Antibody Technique
Proteolysis
Polyacrylamide Gel Electrophoresis
Chickens
DNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Topoisomerase II is a structural component of mitotic chromosome scaffolds. / Earnshaw, William C.; Halligan, Brian; Cooke, Carol A.; Heck, Margarete M S; Liu, Leroy-Fong.

In: Journal of Cell Biology, Vol. 100, No. 5, 01.05.1985, p. 1706-1715.

Research output: Contribution to journalArticle

Earnshaw, William C. ; Halligan, Brian ; Cooke, Carol A. ; Heck, Margarete M S ; Liu, Leroy-Fong. / Topoisomerase II is a structural component of mitotic chromosome scaffolds. In: Journal of Cell Biology. 1985 ; Vol. 100, No. 5. pp. 1706-1715.
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