Titin: Quantitative mass measurements by scanning transmission electron microscopy and structural implications for the sarcomere matrix of skeletal muscle

James F. Hainfeld, Joseph S. Wall, Kuan Wang

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Scanning transmission electron microscopy has been used to investigate mass and linear mass density of native titin-2, a large soluble fragment of intact titin, from rabbit skeletal muscle. Dark field images of unstained, freeze-dried titin-2 appeared as either compact globules or looser and larger balls of string. Direct mass measurements indicated that the compact forms have an average mass of 2.40±0.50 × 106 Da. The mass to length ratio, determined from well-spread portions of titin strands (3-5 nm wide) from the ball of string forms, averaged 2.7±0.9 kDa/nm. Thus a single native intact titin molecule has a calculated contour length of well above ∼1 μm, sufficient to span unidirectionally between the Z line and M line region in a resting-length sarcomere.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalFEBS Letters
Volume234
Issue number1
DOIs
Publication statusPublished - Jul 4 1988
Externally publishedYes

Keywords

  • (Skeletal muscle)
  • Connectin
  • Elastic filament
  • Sarcomere matrix
  • Scanning transmission EM
  • Titin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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