Titin: Major myofibrillar components of striated muscle

K. Wang, J. McClure, A. Tu

Research output: Contribution to journalArticle

418 Citations (Scopus)

Abstract

Electrophoretic analyses of protein components of striated muscle myofibril purified from various vertebrate and invertebrate species revealed that proteins much larger than myosin heavy chain are present in significant amounts. To define possible roles of these heretofore unidentified proteins, we purified a combination of two uncommonly large proteins, designated as titin, from chicken breast myofibrils. Chemical and immunological studies indicated that titin is distinct from myosin, actin and filamin. Specific titin antibody crossreacts with similar protein in both skeletal and cardiac myofibrils of many vertebrate and invertebrate species. Immunofluorescent staining of glycerinated chicken breast myofibrils indicated that titin is present in M lines, Z lines, the junctions of A and I bands, and perhaps throughout the entire A bands. Similar staining studies of myofibrils from other species suggest that titin-like proteins may be organized in all myofibrils according to a common architectural plan. We conclude that titin is a structurally conserved myofibrillar component of vertebrate and invertebrate striated muscles.

Original languageEnglish
Pages (from-to)3698-3702
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number8
DOIs
Publication statusPublished - 1979
Externally publishedYes

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