Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

Rong Sen Yang, Huei Ping Tzeng, Feng Ming Ho, Chia Chi Chuang, Bo Lin Chen, Chun Fa Huang, Ya Wen Chen, Ruei Ming Chen, Shing Hwa Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.

Original languageEnglish
Pages (from-to)3258-3264
Number of pages7
JournalActa Biomaterialia
Volume5
Issue number8
DOIs
Publication statusPublished - Oct 2009

Keywords

  • Aorta
  • Endothelial cells
  • eNOS
  • Titanium alloy implants

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Yang, R. S., Tzeng, H. P., Ho, F. M., Chuang, C. C., Chen, B. L., Huang, C. F., Chen, Y. W., Chen, R. M., & Liu, S. H. (2009). Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway. Acta Biomaterialia, 5(8), 3258-3264. https://doi.org/10.1016/j.actbio.2009.05.006