Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

Rong Sen Yang, Huei Ping Tzeng, Feng Ming Ho, Chia Chi Chuang, Bo Lin Chen, Chun Fa Huang, Ya Wen Chen, Ruei Ming Chen, Shing Hwa Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.

Original languageEnglish
Pages (from-to)3258-3264
Number of pages7
JournalActa Biomaterialia
Volume5
Issue number8
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Titanium
Vasoconstriction
Protein Kinase C
Proteins
Nitric Oxide Synthase Type III
Nitric oxide
Phosphorylation
Titanium alloys
Rats
Aorta
Endothelial cells
Human Umbilical Vein Endothelial Cells
Joint prostheses
Knee prostheses
Mitogen-Activated Protein Kinase 3
Orthopedics
Phenylephrine
Joint Prosthesis
Knee Prosthesis
Protein C Inhibitor

Keywords

  • Aorta
  • Endothelial cells
  • eNOS
  • Titanium alloy implants

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Yang, R. S., Tzeng, H. P., Ho, F. M., Chuang, C. C., Chen, B. L., Huang, C. F., ... Liu, S. H. (2009). Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway. Acta Biomaterialia, 5(8), 3258-3264. https://doi.org/10.1016/j.actbio.2009.05.006

Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway. / Yang, Rong Sen; Tzeng, Huei Ping; Ho, Feng Ming; Chuang, Chia Chi; Chen, Bo Lin; Huang, Chun Fa; Chen, Ya Wen; Chen, Ruei Ming; Liu, Shing Hwa.

In: Acta Biomaterialia, Vol. 5, No. 8, 10.2009, p. 3258-3264.

Research output: Contribution to journalArticle

Yang, RS, Tzeng, HP, Ho, FM, Chuang, CC, Chen, BL, Huang, CF, Chen, YW, Chen, RM & Liu, SH 2009, 'Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway', Acta Biomaterialia, vol. 5, no. 8, pp. 3258-3264. https://doi.org/10.1016/j.actbio.2009.05.006
Yang, Rong Sen ; Tzeng, Huei Ping ; Ho, Feng Ming ; Chuang, Chia Chi ; Chen, Bo Lin ; Huang, Chun Fa ; Chen, Ya Wen ; Chen, Ruei Ming ; Liu, Shing Hwa. / Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway. In: Acta Biomaterialia. 2009 ; Vol. 5, No. 8. pp. 3258-3264.
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