Tissue microarray analysis of interleukin-20 expression

Chung-Hsi Hsing, Chung Liang Ho, Lih Yun Chang, Yi Lin Lee, Shih Sung Chuang, Ming Shi Chang

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Knowledge about the biological functions and clinical implications of interleukin (IL)-20, a recently discovered cytokine in the IL-10 family, is still incomplete. Our aim was to determine the distribution of IL-20 expression and to delineate the cell types that express IL-20 in healthy and neoplastic tissue, because this information will significantly affect the exploration of its pathophysiological roles. We used tissue microarray technology and an immunohistochemical survey using an anti-IL-20 monoclonal antibody to examine IL-20 expression in 36 non-neoplastic and 14 neoplastic tissues. IL-20 protein was positively stained in 30 non-neoplastic tissue types and five major cell types: epithelial cells, myoepithelial cells, endothelial cells, macrophages, and skeletal muscle cells. We also found that several types of tumor cells stained positive for IL-20, especially in squamous cell carcinoma of the skin, tongue, esophagus, and lung. Our data provide valuable references for further investigation of the biological functions and clinical implications of IL-20 in humans.

Original languageEnglish
Pages (from-to)44-52
Number of pages9
JournalCytokine
Volume35
Issue number1-2
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Keywords

  • Cytokines
  • Interleukin-20
  • Tissue microarray

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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