Tissue-engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF-β1 in platelet-rich plasma

Wei Hong Chen, Wen Cheng Lo, Jie Jen Lee, Ching Hua Su, Che Tong Lin, Hen Yu Liu, Tsou Wen Lin, Wei Chao Lin, Te Yang Huang, Win Ping Deng

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Human intervertebral disc (IVD) degeneration often initiated from the human nucleus pulposus (hNP) with aging leading to IVD destruction and extracellular matrix (ECM) depletion. Previously, we have successfully employed transforming growth factor-β1 (TGF-β1) to promote chondrogenesis of mesenchymal progenitor cells (MPCs) and immortalized human mesenchymal stem cells. In this study, we examine the role of TGF-β1 in platelet-rich plasma (PRP) on disc regeneration, including proliferation, redifferentiation, and the reconstitution of tissue-engineered NP. hNP cells were isolated from volunteers with different ages and cultured in the presence of PRP. We found that the most effective concentration for hNP proliferation was 1 ng/ml TGF-β1 in PRP, which was further applied in the following experiments. hNP cell proliferation in all age groups were increased time-dependently by PRP and cell morphologies showed aggregation. The mRNA of Sox9, type II collagen, and aggrecan were all significantly upregulated by PRP through RT-PCR. Glycosaminoglycan (GAG) accumulation reached the highest value at day 7 and continued to day 9 culture. PRP promoted NP regeneration via the Smad pathway was also determined and highly activated p-Smad2/3 at 30 min and continuously sustained to 120 min. Immunostaining of type II collagen indicates that PRP participates in chondrogenesis of tissue-engineered NP with collagen scaffolds. We concluded that growth factors in PRP can effectively react as a growth factor cocktail to induce hNP proliferation and differentiation, and also promote tissue-engineered NP formation. These findings are the first to demonstrate that PRP might be a therapeutic candidate for prevention of disc degeneration.

Original languageEnglish
Pages (from-to)744-754
Number of pages11
JournalJournal of Cellular Physiology
Volume209
Issue number3
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Chondrogenesis
Platelet-Rich Plasma
Intervertebral Disc
Transforming Growth Factors
Platelets
Tissue
Plasmas
Intervertebral Disc Degeneration
Collagen Type II
Mesenchymal Stromal Cells
Regeneration
Intercellular Signaling Peptides and Proteins
Nucleus Pulposus
Aggrecans
Cell proliferation
Plasma Cells
Glycosaminoglycans
Stem cells
Scaffolds
Extracellular Matrix

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Tissue-engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF-β1 in platelet-rich plasma. / Chen, Wei Hong; Lo, Wen Cheng; Lee, Jie Jen; Su, Ching Hua; Lin, Che Tong; Liu, Hen Yu; Lin, Tsou Wen; Lin, Wei Chao; Huang, Te Yang; Deng, Win Ping.

In: Journal of Cellular Physiology, Vol. 209, No. 3, 12.2006, p. 744-754.

Research output: Contribution to journalArticle

Chen, Wei Hong ; Lo, Wen Cheng ; Lee, Jie Jen ; Su, Ching Hua ; Lin, Che Tong ; Liu, Hen Yu ; Lin, Tsou Wen ; Lin, Wei Chao ; Huang, Te Yang ; Deng, Win Ping. / Tissue-engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF-β1 in platelet-rich plasma. In: Journal of Cellular Physiology. 2006 ; Vol. 209, No. 3. pp. 744-754.
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AU - Huang, Te Yang

AU - Deng, Win Ping

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