Tissue bioengineering for surgical bleb defects: An animal study

Wei Cherng Hsu, Robert Ritch, Theodore Krupin, Henry Shen Lih Chen

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


Background: To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect. Methods: The right eyes of 30 female New Zealand albino rabbits underwent trabeculectomy with OloGen implanted subconjunctivally on top of the scleral flap, while six right eyes received trabeculectomy without the implant to serve as a control group. A 1-2 mm diameter circular conjunctival defect was created in all eyes. Six rabbits in the group receiving the implant were sacrificed on days 3, 5, 7, 21, and 28. Rabbits in the control group were sacrificed on day 28. Perkins applanation tonometry, Seidel test and measurement of both the extent of the conjunctival defect and the anterior chamber depth were performed. Enucleated eyes were fixed in 4% formaldehyde and stained with hematoxylin and eosin (H&E) for general histological observation, and with Sirius and Fast-green stains to assess collagen deposition and cell migration. Results: Seidel tests were negative for all operated and control eyes. No flat anterior chamber occurred in either group. With the exception of days 5 and 7, post-operative mean IOP difference is significant in both groups, (P>0.05 for day 5, 7 and P

Original languageEnglish
Pages (from-to)709-717
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Issue number5
Publication statusPublished - May 2008


  • Biodegradable
  • Collagen matrix
  • Glaucoma
  • Implant
  • Tissue engineering
  • Trabeculectomy
  • Wound healing

ASJC Scopus subject areas

  • Ophthalmology


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