TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Yu Jui Fan, Horn Jiunn Sheen, Zheng Yu Chen, Yi Hsing Liu, Jing Fa Tsai, Kuang Chong Wu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

This paper reports the in situ detection of C-reactive protein (CRP) and antibody-coated nanobead interaction by measuring the variation of the Brownian diffusion of the nanobeads. The measurements are carried out by using total internal reflective fluorescence-enhanced (TIRF-enhanced) micro-particle-tracking velocimetry (micro-PTV), which is highly sensitive for analyzing nanobead movements in the near-wall region. The characteristics of evanescent wave penetration of various liquid samples, which have different refractive indices, are investigated for nanobead illumination. The Brownian velocities of the nanobeads in various concentrations of CRPs within glycerol solution and serum are measured in real time. The detectable CRP concentration range of 1–6 mg/ml in human serum is found, and the detecting process can be finished in 10 min. From the results, the dissociation constant of CRP and anti-CRP in various viscosity solutions can be determined through the developed kinetic analysis.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalMicrofluidics and Nanofluidics
Volume19
Issue number1
DOIs
Publication statusPublished - Jul 15 2015
Externally publishedYes

Keywords

  • Brownian velocity
  • CRP
  • Dissociation constant
  • Micro-PTV
  • TIRF

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

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