Time course alterations of satellite cell events in response to light moderate endurance training in white gastrocnemius muscle of the rat

Z. Y. Cai, C. C. Hsu, M. C. Hsu, M. C. Huang, C. P. Yang, S. L. Lee, W. C. Lin, B. Su

Research output: Contribution to journalArticle

Abstract

This study investigated satellite cells and their related molecular events adapted to light moderate endurance training in the white gastrocnemius muscle of the rat. The white gastrocnemius muscle of male Sprague-Dawley rats that had been trained for 4 weeks and 8 weeks, with control rats being analysed alongside them, was selected for analysis (n=3 per group). The training protocol consisted of treadmill running at 20 m min -1 for 30 min on a 0% grade, for 3 days week -1. Immunohistochemical staining coupled with image analysis was used for quantification. To provide deeper insight into the cell layer, 40 sections per rat, corresponding to 120 values per group, were obtained as a mean value for statistical comparison. The results indicated that at week 4, training effects increased the vascular endothelial growth factor (VEGF) content and c-met positive satellite cell numbers. At week 8, the training effect was attenuated for VEGF and c-met satellite cell numbers, but it increased in the muscle fibre area. Additionally, c-met positive satellite cell numbers correlated with VEGF content (r = 0.79, p<0.05). In conclusion, this study suggests that light moderate endurance training could stimulate satellite cell activation that might be related to VEGF signalling. Additionally, the satellite cells activated by moderate endurance training might contribute to slight growth in myocytes.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalBiology of Sport
Volume29
Issue number1
DOIs
Publication statusPublished - 2012

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Vascular Endothelial Growth Factor A
Skeletal Muscle
Light
Cell Count
Running
Muscle Cells
Sprague Dawley Rats
Staining and Labeling
Muscles
Growth

Keywords

  • C-met
  • Exercise
  • VEGF

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Physiology (medical)

Cite this

Time course alterations of satellite cell events in response to light moderate endurance training in white gastrocnemius muscle of the rat. / Cai, Z. Y.; Hsu, C. C.; Hsu, M. C.; Huang, M. C.; Yang, C. P.; Lee, S. L.; Lin, W. C.; Su, B.

In: Biology of Sport, Vol. 29, No. 1, 2012, p. 51-56.

Research output: Contribution to journalArticle

Cai, Z. Y. ; Hsu, C. C. ; Hsu, M. C. ; Huang, M. C. ; Yang, C. P. ; Lee, S. L. ; Lin, W. C. ; Su, B. / Time course alterations of satellite cell events in response to light moderate endurance training in white gastrocnemius muscle of the rat. In: Biology of Sport. 2012 ; Vol. 29, No. 1. pp. 51-56.
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