Tight regulation of a timed nuclear import wave of EKLF by PKCθ and FOE during Pro-E to Baso-E transition

Yu Chiau Shyu, Tung Liang Lee, Xin Chen, Pang Hung Hsu, Shau Ching Wen, Yi Wei Liaw, Chi Huan Lu, Po Yen Hsu, Mu Jie Lu, JauLang Hwang, Ming Daw Tsai, Ming Jing Hwang, Jim Ray Chen, Che Kun James Shen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Erythropoiesis is a highly regulated process during which BFU-E are differentiated into RBCs through CFU-E, Pro-E, PolyCh-E, OrthoCh-E, and reticulocyte stages. Uniquely, most erythroid-specific genes are activated during the Pro-E to Baso-E transition. We show that a wave of nuclear import of the erythroid-specific transcription factor EKLF occurs during the Pro-E to Baso-E transition. We further demonstrate that this wave results from a series of finely tuned events, including timed activation of PKCθ, phosphorylation of EKLF at S68 by P-PKCθ(S676), and sumoylation of EKLF at K74. The latter EKLF modifications modulate its interactions with a cytoplasmic ankyrin-repeat-protein FOE and importinβ1, respectively. The role of FOE in the control of EKLF nuclear import is further supported by analysis of the subcellular distribution patterns of EKLF in FOE-knockout mice. This study reveals the regulatory mechanisms of the nuclear import of EKLF, which may also be utilized in the nuclear import of other factors.

Original languageEnglish
Pages (from-to)409-422
Number of pages14
JournalDevelopmental Cell
Volume28
Issue number4
DOIs
Publication statusPublished - Feb 24 2014
Externally publishedYes

Fingerprint

Cell Nucleus Active Transport
Erythroid Precursor Cells
Ankyrin Repeat
Sumoylation
Phosphorylation
Erythropoiesis
Reticulocytes
erythroid Kruppel-like factor
Knockout Mice
Transcription Factors
Genes
Chemical activation

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Shyu, Y. C., Lee, T. L., Chen, X., Hsu, P. H., Wen, S. C., Liaw, Y. W., ... James Shen, C. K. (2014). Tight regulation of a timed nuclear import wave of EKLF by PKCθ and FOE during Pro-E to Baso-E transition. Developmental Cell, 28(4), 409-422. https://doi.org/10.1016/j.devcel.2014.01.007

Tight regulation of a timed nuclear import wave of EKLF by PKCθ and FOE during Pro-E to Baso-E transition. / Shyu, Yu Chiau; Lee, Tung Liang; Chen, Xin; Hsu, Pang Hung; Wen, Shau Ching; Liaw, Yi Wei; Lu, Chi Huan; Hsu, Po Yen; Lu, Mu Jie; Hwang, JauLang; Tsai, Ming Daw; Hwang, Ming Jing; Chen, Jim Ray; James Shen, Che Kun.

In: Developmental Cell, Vol. 28, No. 4, 24.02.2014, p. 409-422.

Research output: Contribution to journalArticle

Shyu, YC, Lee, TL, Chen, X, Hsu, PH, Wen, SC, Liaw, YW, Lu, CH, Hsu, PY, Lu, MJ, Hwang, J, Tsai, MD, Hwang, MJ, Chen, JR & James Shen, CK 2014, 'Tight regulation of a timed nuclear import wave of EKLF by PKCθ and FOE during Pro-E to Baso-E transition', Developmental Cell, vol. 28, no. 4, pp. 409-422. https://doi.org/10.1016/j.devcel.2014.01.007
Shyu, Yu Chiau ; Lee, Tung Liang ; Chen, Xin ; Hsu, Pang Hung ; Wen, Shau Ching ; Liaw, Yi Wei ; Lu, Chi Huan ; Hsu, Po Yen ; Lu, Mu Jie ; Hwang, JauLang ; Tsai, Ming Daw ; Hwang, Ming Jing ; Chen, Jim Ray ; James Shen, Che Kun. / Tight regulation of a timed nuclear import wave of EKLF by PKCθ and FOE during Pro-E to Baso-E transition. In: Developmental Cell. 2014 ; Vol. 28, No. 4. pp. 409-422.
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