Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells

Yu Tang Chin, Po Li Wei, Yih Ho, André Wendindondé Nana, Chun A. Changou, Yi Ru Chen, Yu Chen SH Yang, Meng Ti Hsieh, Aleck Hercbergs, Paul J. Davis, Ya Jung Shih, Hung Yun Lin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Thyroid hormone, l-thyroxine (T4), has been shown to promote ovarian cancer cell proliferation via a receptor on plasma membrane integrin αvβ3 and to induce the activation of ERK1/2 and expression of programmed death-ligand 1 (PD-L1) in cancer cells. In contrast, resveratrol binds to integrin αvβ3 at a discrete site and induces p53-dependent antiproliferation in malignant neoplastic cells. The mechanism of resveratrol action requires nuclear accumulation of inducible cyclooxygenase (COX)-2 and its complexation with phosphorylated ERK1/2. In this study, we examined the mechanism by which T4 impairs resveratrol-induced antiproliferation in human ovarian cancer cells and found that T4 inhibited resveratrol-induced nuclear accumulation of COX-2. Furthermore, T4 increased expression and cytoplasmic accumulation of PD-L1, which in turn acted to retain inducible COX-2 in the cytoplasm. Knockdown of PD-L1 by small hairpin RNA (shRNA) relieved the inhibitory effect of T4 on resveratrol-induced nuclear accumulation of COX-2- and COX-2/p53-dependent gene expression. Thus, T4 inhibits COX-2-dependent apoptosis in ovarian cancer cells by retaining inducible COX-2 with PD-L1 in the cytoplasm. These findings provide new insights into the antagonizing effect of T4 on resveratrol’s anticancer properties.

Original languageEnglish
Pages (from-to)533-545
Number of pages13
JournalEndocrine-Related Cancer
Volume25
Issue number5
DOIs
Publication statusPublished - May 1 2018

Fingerprint

Cyclooxygenase 2
Thyroxine
Ovarian Neoplasms
Apoptosis
Ligands
Integrins
Cytoplasm
CD4-Positive T-Lymphocytes
p53 Genes
resveratrol
Thyroid Hormones
Small Interfering RNA
Cell Proliferation
Cell Membrane
Gene Expression
Neoplasms

Keywords

  • COX-2
  • L-thyroxine
  • Ovarian cancer
  • PD-L1
  • Resveratrol

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Oncology
  • Endocrinology
  • Cancer Research

Cite this

Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells. / Chin, Yu Tang; Wei, Po Li; Ho, Yih; Nana, André Wendindondé; Changou, Chun A.; Chen, Yi Ru; Yang, Yu Chen SH; Hsieh, Meng Ti; Hercbergs, Aleck; Davis, Paul J.; Shih, Ya Jung; Lin, Hung Yun.

In: Endocrine-Related Cancer, Vol. 25, No. 5, 01.05.2018, p. 533-545.

Research output: Contribution to journalArticle

Chin, YT, Wei, PL, Ho, Y, Nana, AW, Changou, CA, Chen, YR, Yang, YCSH, Hsieh, MT, Hercbergs, A, Davis, PJ, Shih, YJ & Lin, HY 2018, 'Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells', Endocrine-Related Cancer, vol. 25, no. 5, pp. 533-545. https://doi.org/10.1530/ERC-17-0376
Chin, Yu Tang ; Wei, Po Li ; Ho, Yih ; Nana, André Wendindondé ; Changou, Chun A. ; Chen, Yi Ru ; Yang, Yu Chen SH ; Hsieh, Meng Ti ; Hercbergs, Aleck ; Davis, Paul J. ; Shih, Ya Jung ; Lin, Hung Yun. / Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells. In: Endocrine-Related Cancer. 2018 ; Vol. 25, No. 5. pp. 533-545.
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AU - Changou, Chun A.

AU - Chen, Yi Ru

AU - Yang, Yu Chen SH

AU - Hsieh, Meng Ti

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AU - Davis, Paul J.

AU - Shih, Ya Jung

AU - Lin, Hung Yun

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