Thyroid hormone receptor-mediated regulation of the methionine adenosyltransferase 1 gene is associated with cell invasion in hepatoma cell lines

Sheng Ming Wu, Ya Hui Huang, Hsin Yi Lu, Ling Fang Chien, Chau Ting Yeh, Ming Chieh Tsai, Chen Hsin Liao, Wei Jan Chen, Chia Jung Liao, Wan Li Cheng, Kwang Huei Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The thyroid hormone T3 regulates differentiation, growth, and development. We demonstrated that methionine adenosyltransferase 1A (MAT1A) was positively regulated by T3 identified by cDNA microarray previously. The expression of the MAT1A was upregulated by T3 in hepatoma cell lines overexpressing thyroid hormone receptors (TRs). Additionally, these findings indicate that MAT1A may be regulated by CCAAT/enhancer binding protein (C/EBP). The critical role of the C/EBP binding sites was confirmed by the reporter or chromatin immuno-precipitation (ChIP) assay. In addition, C/EBP was upregulated in hepatoma cells after T3 treatment and ectopic expression of MAT1A inhibited cell migration and invasion in J7 hepatoma cells. Conversely, knockdown of MAT1A expression increased cell migration. Together, these findings suggest that the expression of the MAT1A gene is mediated by C/EBP and is indirectly upregulated by T3. Finally, TR was downregulated in a small subset of hepatocellular carcinoma cells concomitantly reduced the expression of C/EBPα and MAT1A.

Original languageEnglish
Pages (from-to)1831-1843
Number of pages13
JournalCellular and Molecular Life Sciences
Volume67
Issue number11
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

Keywords

  • Invasion
  • MAT1A
  • Migration
  • Receptor
  • Thyroid hormone

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Medicine(all)

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  • Cite this

    Wu, S. M., Huang, Y. H., Lu, H. Y., Chien, L. F., Yeh, C. T., Tsai, M. C., Liao, C. H., Chen, W. J., Liao, C. J., Cheng, W. L., & Lin, K. H. (2010). Thyroid hormone receptor-mediated regulation of the methionine adenosyltransferase 1 gene is associated with cell invasion in hepatoma cell lines. Cellular and Molecular Life Sciences, 67(11), 1831-1843. https://doi.org/10.1007/s00018-010-0281-2