Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines

Wen Sen Lee, Mukesh K. Jain, Burak M. Arkonac, Dorothy Zhang, Shyh Yu Shaw, Saori Kashiki, Koji Maemura, Shwu Luan Lee, Norman K. Hollenberg, Mu En Lee, Edgar Haber

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We identified the cell surface glycoprotein Thy-1 on the endothelium of newly formed blood vessels in four models of angiogenesis in adult rats. Anti-Thy-1 staining showed that Thy-1 was upregulated in adventitial blood vessels after balloon injury to the carotid artery. Preabsorption with a rat Thy-1-Ig fusion construct eliminated all immunoreactivity and thus confirmed the specificity of the Thy-1 staining. Thy-1 was also expressed in the endothelium of small blood vessels formed after tumor implantation in the cornea, in periureteral vessels formed after ligation of the renal artery, and in small blood vessels of the uterus formed during pregnancy. In contrast with its expression during adult angiogenesis, Thy-1 was not expressed in the endothelium of blood vessels during embryonic angiogenesis. In vitro, the inflammatory cytokines interleukin-1β and tumor necrosis factor-α upregulated Thy-1 mRNA by 8- and 14-fold, respectively. Vascular endothelial growth factor, basic fibroblast growth factor, transforming growth factor- β, and platelet-derived growth factor-BB had no effect on Thy-1 mRNA. Thus, Thy-1 appears to be a marker of adult but not embryonic angiogenesis. The upregulation of Thy-1 by cytokines but not growth factors indicates the importance of inflammation in the pathogenesis of adult angiogenesis.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalCirculation Research
Volume82
Issue number8
Publication statusPublished - May 4 1998
Externally publishedYes

Fingerprint

Blood Vessels
Cytokines
Endothelium
Carotid Artery Injuries
Staining and Labeling
Adventitia
Messenger RNA
Membrane Glycoproteins
Transforming Growth Factors
Fibroblast Growth Factor 2
Renal Artery
Interleukin-1
Cornea
Vascular Endothelial Growth Factor A
Uterus
Ligation
Intercellular Signaling Peptides and Proteins
Up-Regulation
Tumor Necrosis Factor-alpha
Inflammation

Keywords

  • Angiogenesis
  • Endothelium
  • Inflammation
  • Interleukin-1
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Lee, W. S., Jain, M. K., Arkonac, B. M., Zhang, D., Shaw, S. Y., Kashiki, S., ... Haber, E. (1998). Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines. Circulation Research, 82(8), 845-851.

Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines. / Lee, Wen Sen; Jain, Mukesh K.; Arkonac, Burak M.; Zhang, Dorothy; Shaw, Shyh Yu; Kashiki, Saori; Maemura, Koji; Lee, Shwu Luan; Hollenberg, Norman K.; Lee, Mu En; Haber, Edgar.

In: Circulation Research, Vol. 82, No. 8, 04.05.1998, p. 845-851.

Research output: Contribution to journalArticle

Lee, WS, Jain, MK, Arkonac, BM, Zhang, D, Shaw, SY, Kashiki, S, Maemura, K, Lee, SL, Hollenberg, NK, Lee, ME & Haber, E 1998, 'Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines', Circulation Research, vol. 82, no. 8, pp. 845-851.
Lee WS, Jain MK, Arkonac BM, Zhang D, Shaw SY, Kashiki S et al. Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines. Circulation Research. 1998 May 4;82(8):845-851.
Lee, Wen Sen ; Jain, Mukesh K. ; Arkonac, Burak M. ; Zhang, Dorothy ; Shaw, Shyh Yu ; Kashiki, Saori ; Maemura, Koji ; Lee, Shwu Luan ; Hollenberg, Norman K. ; Lee, Mu En ; Haber, Edgar. / Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines. In: Circulation Research. 1998 ; Vol. 82, No. 8. pp. 845-851.
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