Abstract

Thrombomodulin (TM), a natural anticoagulation factor, maintains circulation homeostasis in endothelial cells. TM has additional roles in modulating inflammation, thrombosis, and carcinogenesis. However, there is little information on the role of TM in the progression and metastasis of ovarian cancer. RNA silencing and cDNA expression vectors were used to manipulate target gene expression in ovarian cancer cells. Cell growth and migration were evaluated by an MTT assay, a wound-healing migration assay, a transwell migration assay, and a biosensor system. In this study, we found that TM silencing may enhance the growth rate of cells. The migratory ability of ovarian cancer cells was enhanced dramatically after TM silencing. TM overexpression in ovarian cells suppressed the proliferation and migration capability. Furthermore, we found that skov-3 cells treated with TM shRNA expressed high levels of fibronectin and vimentin and that the expression of these markers correlated positively with their migratory ability. Our results demonstrate that TM expression may regulate cell growth and migration in ovarian cancer cells. This finding suggests that TM may be a novel prognostic and therapeutic target for ovarian cancer.

Original languageEnglish
Pages (from-to)3743-3751
Number of pages9
JournalTumor Biology
Volume34
Issue number6
DOIs
Publication statusPublished - Dec 2013

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Thrombomodulin
Ovarian Neoplasms
Cell Movement
Growth
Ovarian epithelial cancer
Biosensing Techniques
Vimentin
RNA Interference
Fibronectins
Wound Healing
Small Interfering RNA
Carcinogenesis
Thrombosis
Homeostasis
Endothelial Cells
Complementary DNA
Cell Proliferation
Neoplasm Metastasis
Inflammation
Gene Expression

Keywords

  • Migration
  • Ovarian cancer
  • Thrombomodulin

ASJC Scopus subject areas

  • Cancer Research

Cite this

Thrombomodulin mediates the progression of epithelial ovarian cancer cells. / Chen, Lu Min; Wang, Weu; Lee, Jen Chih; Chiu, Feng Hsiang; Wu, Chun Te; Tai, Cheng Jeng; Wang, Chien Kai; Tai, Chen Jei; Huang, Ming Te; Chang, Yu Jia.

In: Tumor Biology, Vol. 34, No. 6, 12.2013, p. 3743-3751.

Research output: Contribution to journalArticle

Chen, Lu Min ; Wang, Weu ; Lee, Jen Chih ; Chiu, Feng Hsiang ; Wu, Chun Te ; Tai, Cheng Jeng ; Wang, Chien Kai ; Tai, Chen Jei ; Huang, Ming Te ; Chang, Yu Jia. / Thrombomodulin mediates the progression of epithelial ovarian cancer cells. In: Tumor Biology. 2013 ; Vol. 34, No. 6. pp. 3743-3751.
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