Three-vessel middle cerebral artery occlusion model

T. N. Lin, S. T. Chen, Y. Y. He, W. M. Cheung, C. Y. Hsu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In the search for a more reproducible focal ischemic stroke model in the rat, we systematically interrupted blood flow to the right middle cerebral artery (MCA) territory by transient ligation of the right MCA, and both right and left common carotid arteries (CCAs) in succession. The regional CBF in this model was examined by laser Doppler flowmetry and [14C] isopro-pyliodoamphetamine and [14C] iodoantipyrine autoradiographic comparison. The right MCA cortex sustained severe ischemia, with a regional blood flow reduction by 88-92%, whereas only very mild ischemia was noted (reduction of blood flow by 10-20%) outside the right MCA cortex. A ligation-time-dependent focal infarct was consistently noted in the cerebral cortex supplied by the right MCA. The characteristic changes of ischemic necrosis were limited to the cortex, sparing the subcortical structures. The mortality rate was very low (less than 2%). Occlusion of the right MCA alone or together with the right CCA did not consistently cause gross infarction. Permanent ligation of the right MCA and both CCAs had a high mortality. Results from this study showed that a more consistent cortical infarction in the right MCA territory with low mortality can be achieved by interruption of collateral circulation following the right MCA ligation.

Original languageEnglish
Title of host publicationAnimal Models of Acute Neurological Injuries
PublisherHumana Press
Pages141-153
Number of pages13
ISBN (Print)9781603271844
DOIs
Publication statusPublished - 2009

Fingerprint

Middle Cerebral Artery Infarction
Middle Cerebral Artery
arteries
Ligation
Common Carotid Artery
Cerebral Cortex
infarction
carotid arteries
blood flow
cortex
Mortality
ischemia
Ischemia
Collateral Circulation
Laser-Doppler Flowmetry
Regional Blood Flow
Infarction
cerebral cortex
Necrosis
stroke

Keywords

  • Anesthetics
  • Angiogenesis
  • Blood glucose
  • CBF
  • CBV
  • Cortical infarct
  • Edema
  • Gender
  • Ischemic stroke
  • Transient Focal ischemia animal model

ASJC Scopus subject areas

  • Neuroscience(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lin, T. N., Chen, S. T., He, Y. Y., Cheung, W. M., & Hsu, C. Y. (2009). Three-vessel middle cerebral artery occlusion model. In Animal Models of Acute Neurological Injuries (pp. 141-153). Humana Press. https://doi.org/10.1007/978-1-60327-185-1_12

Three-vessel middle cerebral artery occlusion model. / Lin, T. N.; Chen, S. T.; He, Y. Y.; Cheung, W. M.; Hsu, C. Y.

Animal Models of Acute Neurological Injuries. Humana Press, 2009. p. 141-153.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lin, TN, Chen, ST, He, YY, Cheung, WM & Hsu, CY 2009, Three-vessel middle cerebral artery occlusion model. in Animal Models of Acute Neurological Injuries. Humana Press, pp. 141-153. https://doi.org/10.1007/978-1-60327-185-1_12
Lin TN, Chen ST, He YY, Cheung WM, Hsu CY. Three-vessel middle cerebral artery occlusion model. In Animal Models of Acute Neurological Injuries. Humana Press. 2009. p. 141-153 https://doi.org/10.1007/978-1-60327-185-1_12
Lin, T. N. ; Chen, S. T. ; He, Y. Y. ; Cheung, W. M. ; Hsu, C. Y. / Three-vessel middle cerebral artery occlusion model. Animal Models of Acute Neurological Injuries. Humana Press, 2009. pp. 141-153
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