Three-dimensional spherical gelatin bubble-based scaffold improves the myotube formation of H9c2 myoblasts

Chieh Mei, Chih Wei Chao, Che Wei Lin, Shing Tak Li, Kuan Han Wu, Kai Chiang Yang, Jiashing Yu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Microenvironmental factors including physical and chemical cues can regulate stem cells as well as terminally differentiated cells to modulate their biological function and differentiation. However, one of the physical cues, the substrate's dimensionality, has not been studied extensively. In this study, the flow-focusing method with a microfluidic device was used to generate gelatin bubbles to fabricate highly ordered three-dimensional (3D) scaffolds. Rat H9c2 myoblasts were seeded into the 3D gelatin bubble-based scaffolds and compared to those grown on 2D gelatin-coating substrates to demonstrate the influences of spatial cues on cell behaviors. Relative to cells on the 2D substrates, the H9c2 myoblasts were featured by a good survival and normal mitochondrial activity but slower cell proliferation within the 3D scaffolds. The cortical actin filaments of H9c2 cells were localized close to the cell membrane when cultured on the 2D substrates, while the F-actins distributed uniformly and occupied most of the cell cytoplasm within the 3D scaffolds. H9c2 myoblasts fused as multinuclear myotubes within the 3D scaffolds without any induction but cells cultured on the 2D substrates had a relatively lower fusion index even differentiation medium was provided. Although there was no difference in actin α 1 and myosin heavy chain 1, H9c2 cells had a higher myogenin messenger RNA level in the 3D scaffolds than those of on the 2D substrates. This study reveals that the dimensionality influences differentiation and fusion of myoblasts.

Original languageEnglish
JournalBiotechnology and Bioengineering
DOIs
Publication statusAccepted/In press - Jan 1 2019

Fingerprint

Myoblasts
Skeletal Muscle Fibers
Gelatin
Scaffolds
Substrates
Cues
Actins
Fusion reactions
Myogenin
Myosin Heavy Chains
Cell proliferation
Cell membranes
Scaffolds (biology)
Stem cells
Actin Cytoskeleton
Rats
Cultured Cells
Cytoplasm
Stem Cells
Cell Proliferation

Keywords

  • dimensionality
  • microenvironment
  • myoblast
  • myotube
  • scaffold

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Three-dimensional spherical gelatin bubble-based scaffold improves the myotube formation of H9c2 myoblasts. / Mei, Chieh; Chao, Chih Wei; Lin, Che Wei; Li, Shing Tak; Wu, Kuan Han; Yang, Kai Chiang; Yu, Jiashing.

In: Biotechnology and Bioengineering, 01.01.2019.

Research output: Contribution to journalArticle

Mei, Chieh ; Chao, Chih Wei ; Lin, Che Wei ; Li, Shing Tak ; Wu, Kuan Han ; Yang, Kai Chiang ; Yu, Jiashing. / Three-dimensional spherical gelatin bubble-based scaffold improves the myotube formation of H9c2 myoblasts. In: Biotechnology and Bioengineering. 2019.
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