Third and second harmonic generation imaging of human articular cartilage

Ming Rung Tsai, Chih-Hwa Chen, Chi Kuang Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Cartilage matrix is damaged in diseased states such as osteoarthritis while adult articular cartilage does not have the capacity to repair structural damage. A least invasive mean to diagnose these diseased states of human articular cartilage with a high spatial resolution is thus highly desired. In this paper we present our harmonic generation microscopic studies on the human articular cartilage samples. Without any staining third and second harmonic generation can provide strong contrast in chondrocytes and collagen matrix respectively. Our study indicates the high capability of harmonic generation microscopy for future articular cartilage disease diagnosis.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7183
DOIs
Publication statusPublished - 2009
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States
Duration: Jan 25 2009Jan 27 2009

Other

OtherMultiphoton Microscopy in the Biomedical Sciences IX
CountryUnited States
CitySan Jose, CA
Period1/25/091/27/09

Fingerprint

Cartilage
cartilage
Second Harmonic Generation
Harmonic generation
harmonic generations
Imaging
Imaging techniques
Harmonic Generation
Osteoarthritis
Collagen
staining
collagens
matrices
Microscopy
Spatial Resolution
Repair
Microscopic examination
High Resolution
Damage
spatial resolution

Keywords

  • 3D microscopy
  • Articular cartilage
  • Chondrocyte
  • Harmonic generation microscopy

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Tsai, M. R., Chen, C-H., & Sun, C. K. (2009). Third and second harmonic generation imaging of human articular cartilage. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7183). [71831V] https://doi.org/10.1117/12.809968

Third and second harmonic generation imaging of human articular cartilage. / Tsai, Ming Rung; Chen, Chih-Hwa; Sun, Chi Kuang.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7183 2009. 71831V.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsai, MR, Chen, C-H & Sun, CK 2009, Third and second harmonic generation imaging of human articular cartilage. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7183, 71831V, Multiphoton Microscopy in the Biomedical Sciences IX, San Jose, CA, United States, 1/25/09. https://doi.org/10.1117/12.809968
Tsai MR, Chen C-H, Sun CK. Third and second harmonic generation imaging of human articular cartilage. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7183. 2009. 71831V https://doi.org/10.1117/12.809968
Tsai, Ming Rung ; Chen, Chih-Hwa ; Sun, Chi Kuang. / Third and second harmonic generation imaging of human articular cartilage. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7183 2009.
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