Therapeutic concentrations of propofol protects mouse macrophages from nitric oxide-induced cell death and apoptosis

Hang Chang, Shih-Ying Tsai, Yi Chang, Ta Liang Chen, Ruei Ming Chen

Research output: Contribution to journalArticle

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Abstract

Purpose: To evaluate the potential effect of a clinically relevant concentration of propofol (PPF) on cell viability and nitric oxide-induced macrophage apoptosis. Methods: Mouse macrophages (cell line Raw 264.7) were cultured and incubated with a nitric oxide donor sodium nitroprusside (SNP), PPF, and a combination of PPF and SNP for one, six and 24 hr. Cell viability was determined by the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Apoptotic cells were determined by analyzing the percentages of sub-GI phase in macrophages. The amounts of nitric oxide were assayed. Results: The amounts of nitric oxide in macrophages were increased with time when incubated with SNP (P <0.05). Simultaneously, SNP caused cell death of macrophages in a concentration-and time-dependent manner (P <0.05). PPF per se did not alter the amount of basal and SNP-provided nitric oxide in macrophages. A therapeutic concentration of PPF (30 μM) exhibited no cytotoxicity. After incubation with SNP for one and six hours, PPF could completely or partially block nitric oxide-induced cell death, respectively (P <0.05). Administration of SNP to macrophages resulted in a time-dependent pattern of increase of apoptotic cells (P <0.05). Similar to the results of the cell viability analyses, PPF was able to protect macrophages from nitric oxide-induced apoptosis in one and six hour-treated groups (P <0.05) but not in the 24 hr treated group. Conclusion: PPF, at a therapeutic concentration, can protect mouse macrophages in vitro from nitric oxide-induced cell apoptosis as well as cell death.

Original languageEnglish
Pages (from-to)477-480
Number of pages4
JournalCanadian Journal of Anesthesia
Volume49
Issue number5
Publication statusPublished - 2002

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Propofol
Nitroprusside
Nitric Oxide
Cell Death
Macrophages
Apoptosis
Cell Survival
Therapeutics
Nitric Oxide Donors
Cell Line

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Therapeutic concentrations of propofol protects mouse macrophages from nitric oxide-induced cell death and apoptosis. / Chang, Hang; Tsai, Shih-Ying; Chang, Yi; Chen, Ta Liang; Chen, Ruei Ming.

In: Canadian Journal of Anesthesia, Vol. 49, No. 5, 2002, p. 477-480.

Research output: Contribution to journalArticle

Chang, Hang ; Tsai, Shih-Ying ; Chang, Yi ; Chen, Ta Liang ; Chen, Ruei Ming. / Therapeutic concentrations of propofol protects mouse macrophages from nitric oxide-induced cell death and apoptosis. In: Canadian Journal of Anesthesia. 2002 ; Vol. 49, No. 5. pp. 477-480.
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abstract = "Purpose: To evaluate the potential effect of a clinically relevant concentration of propofol (PPF) on cell viability and nitric oxide-induced macrophage apoptosis. Methods: Mouse macrophages (cell line Raw 264.7) were cultured and incubated with a nitric oxide donor sodium nitroprusside (SNP), PPF, and a combination of PPF and SNP for one, six and 24 hr. Cell viability was determined by the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Apoptotic cells were determined by analyzing the percentages of sub-GI phase in macrophages. The amounts of nitric oxide were assayed. Results: The amounts of nitric oxide in macrophages were increased with time when incubated with SNP (P <0.05). Simultaneously, SNP caused cell death of macrophages in a concentration-and time-dependent manner (P <0.05). PPF per se did not alter the amount of basal and SNP-provided nitric oxide in macrophages. A therapeutic concentration of PPF (30 μM) exhibited no cytotoxicity. After incubation with SNP for one and six hours, PPF could completely or partially block nitric oxide-induced cell death, respectively (P <0.05). Administration of SNP to macrophages resulted in a time-dependent pattern of increase of apoptotic cells (P <0.05). Similar to the results of the cell viability analyses, PPF was able to protect macrophages from nitric oxide-induced apoptosis in one and six hour-treated groups (P <0.05) but not in the 24 hr treated group. Conclusion: PPF, at a therapeutic concentration, can protect mouse macrophages in vitro from nitric oxide-induced cell apoptosis as well as cell death.",
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