The zebrafish erythropoietin: Functional identification and biochemical characterization

Cheng Ying Chu, Chia Hsiung Cheng, Gen D. Chen, Yi Chung Chen, Chin Chun Hung, Kai Y. Huang, Chang J. Huang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In the present study, the zebrafish epo cDNA was cloned. The encoded protein displays 90%, 55% and 32% identity to the Epo from carp, fugu and human, respectively. Through RT-PCR, the expression of zepo mRNA was mainly in the heart and liver. In the COS-1 cell transfection experiments, the recombinant zEpo-HA protein was efficiently secreted into the culture medium as a glycoprotein and the carbohydrate moiety can be cleaved by the treatment of peptide-N-glycosidase F (PNGase F). Using the morpholino approach, we showed that zepo morphants displayed severe anemia leading to high mortality during development. Such an effect can be significantly rescued by zepo RNA. Furthermore, in the absence of functional zEpo, the expression of specific markers for adult globin genes, such as αA1- and βA1-globin, but not the embryonic βe1-globin, was affected.

Original languageEnglish
Pages (from-to)4265-4271
Number of pages7
JournalFEBS Letters
Volume581
Issue number22
DOIs
Publication statusPublished - Sep 4 2007

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Globins
Zebrafish
Erythropoietin
Takifugu
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Morpholinos
Carps
COS Cells
Liver
Transfection
Culture Media
Anemia
Glycoproteins
Proteins
Complementary DNA
Genes
Display devices
Carbohydrates
RNA
Polymerase Chain Reaction

Keywords

  • Erythropoietin
  • Globin staining
  • Glycoprotein
  • Morpholino oligonucleotide
  • Secretion
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The zebrafish erythropoietin : Functional identification and biochemical characterization. / Chu, Cheng Ying; Cheng, Chia Hsiung; Chen, Gen D.; Chen, Yi Chung; Hung, Chin Chun; Huang, Kai Y.; Huang, Chang J.

In: FEBS Letters, Vol. 581, No. 22, 04.09.2007, p. 4265-4271.

Research output: Contribution to journalArticle

Chu, Cheng Ying ; Cheng, Chia Hsiung ; Chen, Gen D. ; Chen, Yi Chung ; Hung, Chin Chun ; Huang, Kai Y. ; Huang, Chang J. / The zebrafish erythropoietin : Functional identification and biochemical characterization. In: FEBS Letters. 2007 ; Vol. 581, No. 22. pp. 4265-4271.
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