The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma

H. J. Harn, L. I. Ho, C. P. Yu, M. W. Wang, H. S. Lee, J. J. Lin, W. H. Lee, N. R. Isola, D. L. Cooper

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The interaction of the cell surface receptor CD44 molecular with its ligands (addressin, extracellular matrix etc.,) plays an important role in fulfilling the lymphocyte homing and immune reaction. Recently alternatively spliced products of CD44 gene are found to be involved in tumor metastasis as well. Our report found that CD44 prototype RNA (CD44S) was present in all five tumor cell lines. Isoform CD44 RNA (CD44V) was recognized in three metastasized hepatocellular carcinoma cell lines, J5, HCC36, HEP38. In addition, the J5 CD44 RNA isoform expressed two distinct trancripts which are of the same size as MDA-231 breast tumor cell line. The MDA-231 CD44 RNA variant (CD44V) has been confirmed to contain metastasis domain 4 and 5. it is implicated that the alternative RNA splicing may also play a major role in hepatocellular carcinoma metastasis.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume32
Issue number2
Publication statusPublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

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    Harn, H. J., Ho, L. I., Yu, C. P., Wang, M. W., Lee, H. S., Lin, J. J., Lee, W. H., Isola, N. R., & Cooper, D. L. (1994). The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma. Biochemistry and Molecular Biology International, 32(2), 233-238.