The use of chitosan to enhance photodynamic inactivation against Candida albicans and its drug-resistant clinical isolates

Hsiung-Fei Chien, Chueh Pin Chen, Yee Chun Chen, Po Han Chang, Tsuimin Tsai, Chin Tin Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Drug-resistant Candida infection is a major health concern among immunocompromised patients. Antimicrobial photodynamic inactivation (PDI) was introduced as an alternative treatment for local infections. Although Candida (C.) has demonstrated susceptibility to PDI, high doses of photosensitizer (PS) and light energy are required, which may be harmful to eukaryotic human cells. This study explores the capacity of chitosan, a polycationic biopolymer, to increase the efficacy of PDI against C. albicans, as well as fluconazole-resistant clinical isolates in planktonic or biofilm states. Chitosan was shown to effectively augment the effect of PDI mediated by toluidine blue O (TBO) against C. albicans that were incubated with chitosan for 30 min following PDI. Chitosan at concentrations as low as 0.25% eradicated C. albicans; however, without PDI treatment, chitosan alone did not demonstrate significant antimicrobial activity within the 30 min of incubation. These results suggest that chitosan only augmented the fungicidal effect after the cells had been damaged by PDI. Increasing the dosage of chitosan or prolonging the incubation time allowed a reduction in the PDI condition required to completely eradicate C. albicans. These results clearly indicate that combining chitosan with PDI is a promising antimicrobial approach to treat infectious diseases.

Original languageEnglish
Pages (from-to)7445-7456
Number of pages12
JournalInternational Journal of Molecular Sciences
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Candida
Chitosan
Candida albicans
deactivation
drugs
Pharmaceutical Preparations
infectious diseases
Tolonium Chloride
Biopolymers
Photosensitizing Agents
Photosensitizers
Fluconazole
dosage
Immunocompromised Host
Biofilms
biofilms
Eukaryotic Cells
Infection
biopolymers
Dosimetry

Keywords

  • Antimicrobial
  • Candida
  • Chitosan
  • Photodynamic inactivation

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Medicine(all)

Cite this

The use of chitosan to enhance photodynamic inactivation against Candida albicans and its drug-resistant clinical isolates. / Chien, Hsiung-Fei; Chen, Chueh Pin; Chen, Yee Chun; Chang, Po Han; Tsai, Tsuimin; Chen, Chin Tin.

In: International Journal of Molecular Sciences, Vol. 14, No. 4, 04.2013, p. 7445-7456.

Research output: Contribution to journalArticle

Chien, Hsiung-Fei ; Chen, Chueh Pin ; Chen, Yee Chun ; Chang, Po Han ; Tsai, Tsuimin ; Chen, Chin Tin. / The use of chitosan to enhance photodynamic inactivation against Candida albicans and its drug-resistant clinical isolates. In: International Journal of Molecular Sciences. 2013 ; Vol. 14, No. 4. pp. 7445-7456.
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