The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio)

Wei Jen Chang, Jiun Lin Horng, Jia Jiun Yan, Chung Der Hsiao, Pung Pung Hwang

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

H+-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na+ uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing (gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm2 (zgcm2) was found to begin expression in zebrafish embryos at 10 h postfertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole mount in situ hybridization, zgcm2 mRNA signals were found in a group of cells on the zebrafish yolk sac surface initially in the tail bud stage (10 hpf); they had disappeared at 36 hpf and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific colocalization of zgcm2 mRNA in HR cells in zebrafish embryos. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H + activity at the apical surface of HR cells, but it did not affect the occurrence of Na+-K+-ATPase-rich cells. A decrease in the H+-ATPase subunit A (zatp6v1a) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 days after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 days posttransfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume296
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Zebrafish
Neuroglia
Transcription Factors
Embryonic Structures
Morpholinos
Yolk Sac
Messenger RNA
Fresh Water
Fluorescence In Situ Hybridization
Drosophila
In Situ Hybridization
Tail
Testis
Cell Differentiation
Myocardium

Keywords

  • Acid secretion
  • Gills
  • Ion regulation
  • Ionocytes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

@article{1d91323411b74934bfdbb1c205d69ec5,
title = "The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio)",
abstract = "H+-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na+ uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing (gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm2 (zgcm2) was found to begin expression in zebrafish embryos at 10 h postfertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole mount in situ hybridization, zgcm2 mRNA signals were found in a group of cells on the zebrafish yolk sac surface initially in the tail bud stage (10 hpf); they had disappeared at 36 hpf and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific colocalization of zgcm2 mRNA in HR cells in zebrafish embryos. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H + activity at the apical surface of HR cells, but it did not affect the occurrence of Na+-K+-ATPase-rich cells. A decrease in the H+-ATPase subunit A (zatp6v1a) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 days after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 days posttransfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation.",
keywords = "Acid secretion, Gills, Ion regulation, Ionocytes",
author = "Chang, {Wei Jen} and Horng, {Jiun Lin} and Yan, {Jia Jiun} and Hsiao, {Chung Der} and Hwang, {Pung Pung}",
year = "2009",
month = "4",
doi = "10.1152/ajpregu.90973.2008",
language = "English",
volume = "296",
journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio)

AU - Chang, Wei Jen

AU - Horng, Jiun Lin

AU - Yan, Jia Jiun

AU - Hsiao, Chung Der

AU - Hwang, Pung Pung

PY - 2009/4

Y1 - 2009/4

N2 - H+-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na+ uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing (gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm2 (zgcm2) was found to begin expression in zebrafish embryos at 10 h postfertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole mount in situ hybridization, zgcm2 mRNA signals were found in a group of cells on the zebrafish yolk sac surface initially in the tail bud stage (10 hpf); they had disappeared at 36 hpf and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific colocalization of zgcm2 mRNA in HR cells in zebrafish embryos. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H + activity at the apical surface of HR cells, but it did not affect the occurrence of Na+-K+-ATPase-rich cells. A decrease in the H+-ATPase subunit A (zatp6v1a) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 days after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 days posttransfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation.

AB - H+-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na+ uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing (gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm2 (zgcm2) was found to begin expression in zebrafish embryos at 10 h postfertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole mount in situ hybridization, zgcm2 mRNA signals were found in a group of cells on the zebrafish yolk sac surface initially in the tail bud stage (10 hpf); they had disappeared at 36 hpf and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific colocalization of zgcm2 mRNA in HR cells in zebrafish embryos. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H + activity at the apical surface of HR cells, but it did not affect the occurrence of Na+-K+-ATPase-rich cells. A decrease in the H+-ATPase subunit A (zatp6v1a) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 days after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 days posttransfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation.

KW - Acid secretion

KW - Gills

KW - Ion regulation

KW - Ionocytes

UR - http://www.scopus.com/inward/record.url?scp=65949097472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65949097472&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.90973.2008

DO - 10.1152/ajpregu.90973.2008

M3 - Article

C2 - 19193938

AN - SCOPUS:65949097472

VL - 296

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 4

ER -