The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates

Der Cherng Liang; Lee Yung Shih; I. J. Chai; Tsung Hsien Su; Shu Huey Chen; Hsi C. Liu; Akihiro Shimosaka

doi:10.1080/08880010290097152

The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates

Der Cherng Liang, Lee Yung Shih, I. J. Chai, Tsung Hsien Su, Shu Huey Chen, Hsi C. Liu, Akihiro Shimosaka

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 10⁴ MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.

Original language	English
Pages (from-to)	399-405
Number of pages	7
Journal	Pediatric Hematology and Oncology
Volume	19
Issue number	6
DOIs	https://doi.org/10.1080/08880010290097152
Publication status	Published - Sep 2002
Externally published	Yes

Keywords

Cord blood
Erythropoiesis
Myelopoiesis
Premature neonates
Thrombopoietin

ASJC Scopus subject areas

Cancer Research
Management of Technology and Innovation
Hematology
Oncology
Pediatrics, Perinatology, and Child Health

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Liang, D. C., Shih, L. Y., Chai, I. J., Su, T. H., Chen, S. H., Liu, H. C., & Shimosaka, A. (2002). The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates. Pediatric Hematology and Oncology, 19(6), 399-405. https://doi.org/10.1080/08880010290097152

The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates. / Liang, Der Cherng; Shih, Lee Yung; Chai, I. J.; Su, Tsung Hsien; Chen, Shu Huey; Liu, Hsi C.; Shimosaka, Akihiro.

In: Pediatric Hematology and Oncology, Vol. 19, No. 6, 09.2002, p. 399-405.

Research output: Contribution to journal › Article › peer-review

Liang, Der Cherng ; Shih, Lee Yung ; Chai, I. J. ; Su, Tsung Hsien ; Chen, Shu Huey ; Liu, Hsi C. ; Shimosaka, Akihiro. / The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates. In: Pediatric Hematology and Oncology. 2002 ; Vol. 19, No. 6. pp. 399-405.

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abstract = "The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 104 MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.",

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AU - Shimosaka, Akihiro

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