Abstract
The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 104 MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.
| Original language | English |
|---|---|
| Pages (from-to) | 399-405 |
| Number of pages | 7 |
| Journal | Pediatric Hematology and Oncology |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Sep 2002 |
| Externally published | Yes |
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Keywords
- Cord blood
- Erythropoiesis
- Myelopoiesis
- Premature neonates
- Thrombopoietin
ASJC Scopus subject areas
- Cancer Research
- Management of Technology and Innovation
- Hematology
- Oncology
- Pediatrics, Perinatology, and Child Health
Cite this
The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates. / Liang, Der Cherng; Shih, Lee Yung; Chai, I. J.; Su, Tsung Hsien; Chen, Shu Huey; Liu, Hsi C.; Shimosaka, Akihiro.
In: Pediatric Hematology and Oncology, Vol. 19, No. 6, 09.2002, p. 399-405.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates
AU - Liang, Der Cherng
AU - Shih, Lee Yung
AU - Chai, I. J.
AU - Su, Tsung Hsien
AU - Chen, Shu Huey
AU - Liu, Hsi C.
AU - Shimosaka, Akihiro
PY - 2002/9
Y1 - 2002/9
N2 - The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 104 MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.
AB - The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 104 MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.
KW - Cord blood
KW - Erythropoiesis
KW - Myelopoiesis
KW - Premature neonates
KW - Thrombopoietin
UR - http://www.scopus.com/inward/record.url?scp=0036707982&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036707982&partnerID=8YFLogxK
U2 - 10.1080/08880010290097152
DO - 10.1080/08880010290097152
M3 - Article
C2 - 12186362
AN - SCOPUS:0036707982
VL - 19
SP - 399
EP - 405
JO - Pediatric Hematology and Oncology
JF - Pediatric Hematology and Oncology
SN - 0888-0018
IS - 6
ER -