The synergistic effect of thrombopoietin in erythropoiesis with erythropoietin and/or IL-3 and myelopoiesis with G-CSF or IL-3 from umbilical cord blood cells of premature neonates

Der Cherng Liang, Lee Yung Shih, I. J. Chai, Tsung Hsien Su, Shu Huey Chen, Hsi C. Liu, Akihiro Shimosaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 104 MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.

Original languageEnglish
Pages (from-to)399-405
Number of pages7
JournalPediatric Hematology and Oncology
Volume19
Issue number6
DOIs
Publication statusPublished - Sep 2002
Externally publishedYes

Keywords

  • Cord blood
  • Erythropoiesis
  • Myelopoiesis
  • Premature neonates
  • Thrombopoietin

ASJC Scopus subject areas

  • Cancer Research
  • Management of Technology and Innovation
  • Hematology
  • Oncology
  • Pediatrics, Perinatology, and Child Health

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