Abstract
The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokine(s) on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood of premature neonates. Cord blood nonadherent mononuclear cells (MNC) from normal premature neonates were cultured in a methylcellulose system. When cultured with 1 × 104 MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 100 ng/mL, the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .000). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .002) also. TPO synergizes erythropoiesis from cord blood of premature neonates. Likewise, the addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .000) also. TPO synergizes myelopoiesis from cord blood of premature neonates. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood of premature neonates.
Original language | English |
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Pages (from-to) | 399-405 |
Number of pages | 7 |
Journal | Pediatric Hematology and Oncology |
Volume | 19 |
Issue number | 6 |
DOIs | |
Publication status | Published - Sep 2002 |
Externally published | Yes |
Keywords
- Cord blood
- Erythropoiesis
- Myelopoiesis
- Premature neonates
- Thrombopoietin
ASJC Scopus subject areas
- Cancer Research
- Management of Technology and Innovation
- Hematology
- Oncology
- Pediatrics, Perinatology, and Child Health