The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells

Der Cherng Liang, L. Y. Shih, M. C. Kuo, S. H. Chen, H. C. Liu, A. Shimosaka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with recombinant human erythropoietin (EPO) and/or recombinant human interleukin-3 (IL-3) on erythroid burst formation and granulocyte-macrophage colony formation from human bone marrow (BM). BM cells were from 5 adults and 15 children who underwent bone marrow examination because of a clinical suspicion of malignancy; their bone marrows as well as the complete blood counts were normal and were cultured in a methylcellulose system. TPO has a synergistic effect with EPO or EPO + IL-3 on erythropoiesis of human BM, as the addition of TPO to EPO significantly gave rise to more erythroid bursts (p = 0.0001) and the addition of TPO to EPO + IL-3 might give rise to more erythroid bursts (p = 0.05). TPO also has a synergistic effect with recombinant human granulocyte colony-stimulating factor (G-CSF) on myelopoiesis of human BM, since the addition of TPO to G-CSF gave rise to significantly more granulocyte-macrophage colonies (p = 0.0001). Besides its well-known significant role in megakaryopoiesis, TPO also has synergistic effects on erythropoiesis and myelopoiesis. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalActa Haematologica
Volume102
Issue number3
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Myelopoiesis
Thrombopoietin
Erythropoiesis
Bone Marrow Cells
Erythropoietin
Interleukin-3
Bone Marrow
Granulocyte Colony-Stimulating Factor
Granulocytes
Macrophages
Bone Marrow Examination
Methylcellulose
Blood Cell Count

Keywords

  • Erythropoiesis
  • Erythropoietin
  • Granulocyte colony-stimulating factor
  • Interleukin-3
  • Myelopoiesis
  • Thrombopoietin

ASJC Scopus subject areas

  • Hematology

Cite this

Liang, D. C., Shih, L. Y., Kuo, M. C., Chen, S. H., Liu, H. C., & Shimosaka, A. (1999). The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells. Acta Haematologica, 102(3), 135-139.

The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells. / Liang, Der Cherng; Shih, L. Y.; Kuo, M. C.; Chen, S. H.; Liu, H. C.; Shimosaka, A.

In: Acta Haematologica, Vol. 102, No. 3, 1999, p. 135-139.

Research output: Contribution to journalArticle

Liang, DC, Shih, LY, Kuo, MC, Chen, SH, Liu, HC & Shimosaka, A 1999, 'The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells', Acta Haematologica, vol. 102, no. 3, pp. 135-139.
Liang, Der Cherng ; Shih, L. Y. ; Kuo, M. C. ; Chen, S. H. ; Liu, H. C. ; Shimosaka, A. / The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells. In: Acta Haematologica. 1999 ; Vol. 102, No. 3. pp. 135-139.
@article{295b70758efe41eaacf5d63c7abfccc1,
title = "The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells",
abstract = "We sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with recombinant human erythropoietin (EPO) and/or recombinant human interleukin-3 (IL-3) on erythroid burst formation and granulocyte-macrophage colony formation from human bone marrow (BM). BM cells were from 5 adults and 15 children who underwent bone marrow examination because of a clinical suspicion of malignancy; their bone marrows as well as the complete blood counts were normal and were cultured in a methylcellulose system. TPO has a synergistic effect with EPO or EPO + IL-3 on erythropoiesis of human BM, as the addition of TPO to EPO significantly gave rise to more erythroid bursts (p = 0.0001) and the addition of TPO to EPO + IL-3 might give rise to more erythroid bursts (p = 0.05). TPO also has a synergistic effect with recombinant human granulocyte colony-stimulating factor (G-CSF) on myelopoiesis of human BM, since the addition of TPO to G-CSF gave rise to significantly more granulocyte-macrophage colonies (p = 0.0001). Besides its well-known significant role in megakaryopoiesis, TPO also has synergistic effects on erythropoiesis and myelopoiesis. Copyright (C) 2000 S. Karger AG, Basel.",
keywords = "Erythropoiesis, Erythropoietin, Granulocyte colony-stimulating factor, Interleukin-3, Myelopoiesis, Thrombopoietin",
author = "Liang, {Der Cherng} and Shih, {L. Y.} and Kuo, {M. C.} and Chen, {S. H.} and Liu, {H. C.} and A. Shimosaka",
year = "1999",
language = "English",
volume = "102",
pages = "135--139",
journal = "Acta Haematologica",
issn = "0001-5792",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells

AU - Liang, Der Cherng

AU - Shih, L. Y.

AU - Kuo, M. C.

AU - Chen, S. H.

AU - Liu, H. C.

AU - Shimosaka, A.

PY - 1999

Y1 - 1999

N2 - We sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with recombinant human erythropoietin (EPO) and/or recombinant human interleukin-3 (IL-3) on erythroid burst formation and granulocyte-macrophage colony formation from human bone marrow (BM). BM cells were from 5 adults and 15 children who underwent bone marrow examination because of a clinical suspicion of malignancy; their bone marrows as well as the complete blood counts were normal and were cultured in a methylcellulose system. TPO has a synergistic effect with EPO or EPO + IL-3 on erythropoiesis of human BM, as the addition of TPO to EPO significantly gave rise to more erythroid bursts (p = 0.0001) and the addition of TPO to EPO + IL-3 might give rise to more erythroid bursts (p = 0.05). TPO also has a synergistic effect with recombinant human granulocyte colony-stimulating factor (G-CSF) on myelopoiesis of human BM, since the addition of TPO to G-CSF gave rise to significantly more granulocyte-macrophage colonies (p = 0.0001). Besides its well-known significant role in megakaryopoiesis, TPO also has synergistic effects on erythropoiesis and myelopoiesis. Copyright (C) 2000 S. Karger AG, Basel.

AB - We sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with recombinant human erythropoietin (EPO) and/or recombinant human interleukin-3 (IL-3) on erythroid burst formation and granulocyte-macrophage colony formation from human bone marrow (BM). BM cells were from 5 adults and 15 children who underwent bone marrow examination because of a clinical suspicion of malignancy; their bone marrows as well as the complete blood counts were normal and were cultured in a methylcellulose system. TPO has a synergistic effect with EPO or EPO + IL-3 on erythropoiesis of human BM, as the addition of TPO to EPO significantly gave rise to more erythroid bursts (p = 0.0001) and the addition of TPO to EPO + IL-3 might give rise to more erythroid bursts (p = 0.05). TPO also has a synergistic effect with recombinant human granulocyte colony-stimulating factor (G-CSF) on myelopoiesis of human BM, since the addition of TPO to G-CSF gave rise to significantly more granulocyte-macrophage colonies (p = 0.0001). Besides its well-known significant role in megakaryopoiesis, TPO also has synergistic effects on erythropoiesis and myelopoiesis. Copyright (C) 2000 S. Karger AG, Basel.

KW - Erythropoiesis

KW - Erythropoietin

KW - Granulocyte colony-stimulating factor

KW - Interleukin-3

KW - Myelopoiesis

KW - Thrombopoietin

UR - http://www.scopus.com/inward/record.url?scp=0033383729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033383729&partnerID=8YFLogxK

M3 - Article

C2 - 10692676

AN - SCOPUS:0033383729

VL - 102

SP - 135

EP - 139

JO - Acta Haematologica

JF - Acta Haematologica

SN - 0001-5792

IS - 3

ER -