The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells

Der Cherng Liang, L. Y. Shih, M. C. Kuo, S. H. Chen, H. C. Liu, A. Shimosaka

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with recombinant human erythropoietin (EPO) and/or recombinant human interleukin-3 (IL-3) on erythroid burst formation and granulocyte-macrophage colony formation from human bone marrow (BM). BM cells were from 5 adults and 15 children who underwent bone marrow examination because of a clinical suspicion of malignancy; their bone marrows as well as the complete blood counts were normal and were cultured in a methylcellulose system. TPO has a synergistic effect with EPO or EPO + IL-3 on erythropoiesis of human BM, as the addition of TPO to EPO significantly gave rise to more erythroid bursts (p = 0.0001) and the addition of TPO to EPO + IL-3 might give rise to more erythroid bursts (p = 0.05). TPO also has a synergistic effect with recombinant human granulocyte colony-stimulating factor (G-CSF) on myelopoiesis of human BM, since the addition of TPO to G-CSF gave rise to significantly more granulocyte-macrophage colonies (p = 0.0001). Besides its well-known significant role in megakaryopoiesis, TPO also has synergistic effects on erythropoiesis and myelopoiesis. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalActa Haematologica
Volume102
Issue number3
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Erythropoiesis
  • Erythropoietin
  • Granulocyte colony-stimulating factor
  • Interleukin-3
  • Myelopoiesis
  • Thrombopoietin

ASJC Scopus subject areas

  • Hematology

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