The suppression of peritoneal cellular immunity in women with endometriosis could be restored after gonadotropin releasing hormone agonist treatment

Ming Yih Wu, Kuang Han Chao, Shee Uan Chen, Hsin F.U. Chen, Y. U.Shih Yang, S. U.Cheng Huang, Hong Nerng Ho

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Problem: Our previous study reported that peritoneal natural killer (NK) cytotoxicity and CD3 + CD25 + lymphocyte subpopulation were suppressed in women with advanced endometriosis. Whether these phenomena are general for all stages of endometriosis and whether these alterations could be restored by long-term use of gonadotropin releasing hormone agonist (GnRHa) are further tested in this study. Method: Lymphocyte subpopulations (B cells, NK cells, T cells, and T-cell activation markers such as CD69, HLA-DR, and CD25) and NK cell cytotoxicity of peripheral blood and peritoneal fluid by dual-color flow cytometry and 51 Cr release assay in 30 cases of endometriosis were compared with those in 26 controls. We also compared these changes before and after 6-month treatment with GnRHa for advanced endometriosis. Results: Compared with the controls, only those women with advanced endometriosis showed lower NK cytotoxicity in peritoneal fluid mononuclear cells (PFMC). The CD3 + CD69 + lymphocyte subpopulation decreased in peripheral blood mononuclear cells (PBMC) of advanced endometriosis, while the CD3 + CD25+ lymphocyte subpopulation decreased in both PBMC and PFMC of mild and advanced endometriosis. After GnRHa treatment, the CD3 + CD69 + lymphocyte subpopulation increased in both PBMC and PFMC and the CD3 + CD25 + lymphocyte subpopulation increased in PFMC, but not in PBMC. Conclusion: Impaired local immunological function in the PF of endometriosis was confirmed by this study and the impairments could be restored after long-term GnRHa therapy.

Original languageEnglish
Pages (from-to)510-516
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume35
Issue number6
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

HLA-DR Antigens
Endometriosis
Gonadotropin-Releasing Hormone
Cellular Immunity
Lymphocyte Subsets
Ascitic Fluid
Blood Cells
Therapeutics
Natural Killer Cells
T-Lymphocytes
Flow Cytometry
B-Lymphocytes
Color

Keywords

  • CD25
  • CD69
  • Endometriosis
  • Gonadotropin-releasing hormone agonist
  • HLA-DR
  • NK cell cytotoxicity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

The suppression of peritoneal cellular immunity in women with endometriosis could be restored after gonadotropin releasing hormone agonist treatment. / Wu, Ming Yih; Chao, Kuang Han; Chen, Shee Uan; Chen, Hsin F.U.; Yang, Y. U.Shih; Huang, S. U.Cheng; Ho, Hong Nerng.

In: American Journal of Reproductive Immunology, Vol. 35, No. 6, 01.01.1996, p. 510-516.

Research output: Contribution to journalArticle

Wu, Ming Yih ; Chao, Kuang Han ; Chen, Shee Uan ; Chen, Hsin F.U. ; Yang, Y. U.Shih ; Huang, S. U.Cheng ; Ho, Hong Nerng. / The suppression of peritoneal cellular immunity in women with endometriosis could be restored after gonadotropin releasing hormone agonist treatment. In: American Journal of Reproductive Immunology. 1996 ; Vol. 35, No. 6. pp. 510-516.
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