The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

Keertan Dheda, Jung Su Chang, Jim F. Huggett, Louise U. Kim, Margaret A. Johnson, Alimuddin Zumla, Graham A W Rook

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalTuberculosis
Volume87
Issue number3
DOIs
Publication statusPublished - May 2007
Externally publishedYes

Keywords

  • Half-life
  • Human
  • IL-4
  • IL-4δ2
  • mRNA
  • Tuberculosis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology and Allergy
  • Infectious Diseases
  • veterinary(all)

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