The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

Keertan Dheda, Jung Su Chang, Jim F. Huggett, Louise U. Kim, Margaret A. Johnson, Alimuddin Zumla, Graham A W Rook

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalTuberculosis
Volume87
Issue number3
DOIs
Publication statusPublished - May 2007
Externally publishedYes

Fingerprint

RNA Stability
interleukin-4
Pulmonary Tuberculosis
tuberculosis
Interleukin-4
lungs
Tuberculosis
cytokines
Cytokines
Messenger RNA
actinomycin D
blood
Dactinomycin
prototypes
volunteers
antagonists
Healthy Volunteers
Gene Expression
gene expression
cells

Keywords

  • Half-life
  • Human
  • IL-4
  • IL-4δ2
  • mRNA
  • Tuberculosis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology and Allergy
  • Infectious Diseases
  • veterinary(all)

Cite this

The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not. / Dheda, Keertan; Chang, Jung Su; Huggett, Jim F.; Kim, Louise U.; Johnson, Margaret A.; Zumla, Alimuddin; Rook, Graham A W.

In: Tuberculosis, Vol. 87, No. 3, 05.2007, p. 237-241.

Research output: Contribution to journalArticle

Dheda, Keertan ; Chang, Jung Su ; Huggett, Jim F. ; Kim, Louise U. ; Johnson, Margaret A. ; Zumla, Alimuddin ; Rook, Graham A W. / The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not. In: Tuberculosis. 2007 ; Vol. 87, No. 3. pp. 237-241.
@article{53a30df965ee4ff0940ff4ddfd3a73ba,
title = "The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not",
abstract = "The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p",
keywords = "Half-life, Human, IL-4, IL-4δ2, mRNA, Tuberculosis",
author = "Keertan Dheda and Chang, {Jung Su} and Huggett, {Jim F.} and Kim, {Louise U.} and Johnson, {Margaret A.} and Alimuddin Zumla and Rook, {Graham A W}",
year = "2007",
month = "5",
doi = "10.1016/j.tube.2006.11.001",
language = "English",
volume = "87",
pages = "237--241",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",
number = "3",

}

TY - JOUR

T1 - The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

AU - Dheda, Keertan

AU - Chang, Jung Su

AU - Huggett, Jim F.

AU - Kim, Louise U.

AU - Johnson, Margaret A.

AU - Zumla, Alimuddin

AU - Rook, Graham A W

PY - 2007/5

Y1 - 2007/5

N2 - The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p

AB - The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p

KW - Half-life

KW - Human

KW - IL-4

KW - IL-4δ2

KW - mRNA

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=34047269240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047269240&partnerID=8YFLogxK

U2 - 10.1016/j.tube.2006.11.001

DO - 10.1016/j.tube.2006.11.001

M3 - Article

C2 - 17196882

AN - SCOPUS:34047269240

VL - 87

SP - 237

EP - 241

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

IS - 3

ER -