The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

Keertan Dheda; Jung Su Chang; Jim F. Huggett; Louise U. Kim; Margaret A. Johnson; Alimuddin Zumla; Graham A W Rook

doi:10.1016/j.tube.2006.11.001

The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

Keertan Dheda, Jung Su Chang, Jim F. Huggett, Louise U. Kim, Margaret A. Johnson, Alimuddin Zumla, Graham A W Rook

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p

Original language	English
Pages (from-to)	237-241
Number of pages	5
Journal	Tuberculosis
Volume	87
Issue number	3
DOIs	https://doi.org/10.1016/j.tube.2006.11.001
Publication status	Published - May 2007
Externally published	Yes

Keywords

Half-life
Human
IL-4
IL-4δ2
mRNA
Tuberculosis

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Microbiology
Immunology and Allergy
Infectious Diseases
veterinary(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tube.2006.11.001

Cite this

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title = "The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not",

abstract = "The prototype Th2 cytokine IL-4, and its competitive antagonist IL-4δ2, may be important determinants of outcome in human tuberculosis (TB). However, there are no data on how gene expression of these cytokines is regulated. To evaluate this the stability of IL-4 and IL-4δ2 mRNA after the addition of actinomycin-D, was evaluated in whole blood from subjects with pulmonary TB and uninfected healthy volunteers. The Th2/Th1 (IL-4/IFN-γ) mRNA ratio in unstimulated cells in whole blood was significantly greater in TB subjects than in controls (p",

keywords = "Half-life, Human, IL-4, IL-4δ2, mRNA, Tuberculosis",

author = "Keertan Dheda and Chang, {Jung Su} and Huggett, {Jim F.} and Kim, {Louise U.} and Johnson, {Margaret A.} and Alimuddin Zumla and Rook, {Graham A W}",

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AU - Dheda, Keertan

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AU - Huggett, Jim F.

AU - Kim, Louise U.

AU - Johnson, Margaret A.

AU - Zumla, Alimuddin

AU - Rook, Graham A W

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KW - IL-4

KW - IL-4δ2

KW - mRNA

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The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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