The small subunit ribosomal RNA gene sequence of Pleistophora anguillarum and the use of PCR primers for diagnostic detection of the parasite

Hsu Wei Hung, Chu Fang Lo, Chieh Chih Tseng, Shao En Peng, Chih Ming Chou, Guang Hsiung Kou

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Using the polymerase chain reaction (PCR) and two primers for conserved regions of the small subunit ribosomal RNA (SSU-rRNA) of Microsporidia, a DNA segment about 1,195 base pairs long was amplified from a DNA template prepared from purified spores of the microsporidian species Pleistophora anguillarum. These spores had been isolated from adult eels (Anguilla japonica) with 'Beko Disease.' A comparison of sequence data from other microsporidian species showed P. anguillarum SSU-rRNA to be most similar to Vavraia oncoperae. When juvenile eels were artificially infected with P. anguillarum, enzyme-linked immunosorbent assay could detect a positive infection only 12 days post-infection. However, when suitable PCR primers were used, a DNA fragment of about 0.8 kb was detected from these juvenile eels after only 3 days post-infection. No PCR product was obtained with templates prepared from clinically healthy control animals.

Original languageEnglish
Pages (from-to)556-560
Number of pages5
JournalJournal of Eukaryotic Microbiology
Volume45
Issue number5
Publication statusPublished - Sep 1998
Externally publishedYes

Keywords

  • Anguilla japonica
  • Microsporidia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

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