The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos

Shee Uan Chen, Kuang Han Chao, Ming Yih Wu, Chin Der Chen, Hong Nerng Ho, Yu Shih Yang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy. Design: A preclinical, prospective, randomized, in vitro experiment. Setting: The reproductive unit of a university teaching hospital. Patient(s): Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF. Intervention(s): At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously. Main Outcome Measure(s): Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated. Result(s): The mean time (±SD) for biopsy of each embryo in group I (435 ± 137 seconds) was significantly longer than that in group II (126 ± 32 seconds). The success rates for obtaining an intact blastomere were not different between group I (93%) and group II (97%). The growth capacity to the blastocyst stage was similar among the three groups (34%, 37%, and 38%, respectively). However, the ZP-drilled and biopsied embryos of groups I and II had higher percentages of hatching (34% and 37%, respectively) and complete hatching (17% and 20%, respectively) than did those of group III (10% and 0, respectively). The blastomeres obtained by biopsy in groups I and II were equally fixed (90% vs. 90%, respectively) and shown in fluorescence in situ hybridization (79% vs. 80%, respectively). Conclusion(s): Compared with the conventional method, the simplified technique is more efficient and equally efficacious for blastomere biopsy in preimplantation genetic diagnosis.

Original languageEnglish
Pages (from-to)569-575
Number of pages7
JournalFertility and Sterility
Volume69
Issue number3 SUPPL. 2
Publication statusPublished - Dec 1 1998
Externally publishedYes

Fingerprint

Preimplantation Diagnosis
Blastomeres
Zona Pellucida
Embryo Transfer
Fertilization in Vitro
Prenatal Diagnosis
Fluorescence In Situ Hybridization
Chromosome Aberrations
Embryonic Development
Embryonic Structures
Prospective Studies
Biopsy
Pregnancy
Needle Biopsy
Blastocyst
Growth
Teaching Hospitals
Outcome Assessment (Health Care)
Control Groups

Keywords

  • Blastomere biopsy
  • Fluorescence in situ hybridization
  • Preimplantation genetic diagnosis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos. / Chen, Shee Uan; Chao, Kuang Han; Wu, Ming Yih; Chen, Chin Der; Ho, Hong Nerng; Yang, Yu Shih.

In: Fertility and Sterility, Vol. 69, No. 3 SUPPL. 2, 01.12.1998, p. 569-575.

Research output: Contribution to journalArticle

Chen, Shee Uan ; Chao, Kuang Han ; Wu, Ming Yih ; Chen, Chin Der ; Ho, Hong Nerng ; Yang, Yu Shih. / The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos. In: Fertility and Sterility. 1998 ; Vol. 69, No. 3 SUPPL. 2. pp. 569-575.
@article{610ad3744fb64017ac1758eba97b4162,
title = "The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos",
abstract = "Objective: To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy. Design: A preclinical, prospective, randomized, in vitro experiment. Setting: The reproductive unit of a university teaching hospital. Patient(s): Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF. Intervention(s): At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously. Main Outcome Measure(s): Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated. Result(s): The mean time (±SD) for biopsy of each embryo in group I (435 ± 137 seconds) was significantly longer than that in group II (126 ± 32 seconds). The success rates for obtaining an intact blastomere were not different between group I (93{\%}) and group II (97{\%}). The growth capacity to the blastocyst stage was similar among the three groups (34{\%}, 37{\%}, and 38{\%}, respectively). However, the ZP-drilled and biopsied embryos of groups I and II had higher percentages of hatching (34{\%} and 37{\%}, respectively) and complete hatching (17{\%} and 20{\%}, respectively) than did those of group III (10{\%} and 0, respectively). The blastomeres obtained by biopsy in groups I and II were equally fixed (90{\%} vs. 90{\%}, respectively) and shown in fluorescence in situ hybridization (79{\%} vs. 80{\%}, respectively). Conclusion(s): Compared with the conventional method, the simplified technique is more efficient and equally efficacious for blastomere biopsy in preimplantation genetic diagnosis.",
keywords = "Blastomere biopsy, Fluorescence in situ hybridization, Preimplantation genetic diagnosis",
author = "Chen, {Shee Uan} and Chao, {Kuang Han} and Wu, {Ming Yih} and Chen, {Chin Der} and Ho, {Hong Nerng} and Yang, {Yu Shih}",
year = "1998",
month = "12",
day = "1",
language = "English",
volume = "69",
pages = "569--575",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier",
number = "3 SUPPL. 2",

}

TY - JOUR

T1 - The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos

AU - Chen, Shee Uan

AU - Chao, Kuang Han

AU - Wu, Ming Yih

AU - Chen, Chin Der

AU - Ho, Hong Nerng

AU - Yang, Yu Shih

PY - 1998/12/1

Y1 - 1998/12/1

N2 - Objective: To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy. Design: A preclinical, prospective, randomized, in vitro experiment. Setting: The reproductive unit of a university teaching hospital. Patient(s): Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF. Intervention(s): At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously. Main Outcome Measure(s): Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated. Result(s): The mean time (±SD) for biopsy of each embryo in group I (435 ± 137 seconds) was significantly longer than that in group II (126 ± 32 seconds). The success rates for obtaining an intact blastomere were not different between group I (93%) and group II (97%). The growth capacity to the blastocyst stage was similar among the three groups (34%, 37%, and 38%, respectively). However, the ZP-drilled and biopsied embryos of groups I and II had higher percentages of hatching (34% and 37%, respectively) and complete hatching (17% and 20%, respectively) than did those of group III (10% and 0, respectively). The blastomeres obtained by biopsy in groups I and II were equally fixed (90% vs. 90%, respectively) and shown in fluorescence in situ hybridization (79% vs. 80%, respectively). Conclusion(s): Compared with the conventional method, the simplified technique is more efficient and equally efficacious for blastomere biopsy in preimplantation genetic diagnosis.

AB - Objective: To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy. Design: A preclinical, prospective, randomized, in vitro experiment. Setting: The reproductive unit of a university teaching hospital. Patient(s): Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF. Intervention(s): At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously. Main Outcome Measure(s): Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated. Result(s): The mean time (±SD) for biopsy of each embryo in group I (435 ± 137 seconds) was significantly longer than that in group II (126 ± 32 seconds). The success rates for obtaining an intact blastomere were not different between group I (93%) and group II (97%). The growth capacity to the blastocyst stage was similar among the three groups (34%, 37%, and 38%, respectively). However, the ZP-drilled and biopsied embryos of groups I and II had higher percentages of hatching (34% and 37%, respectively) and complete hatching (17% and 20%, respectively) than did those of group III (10% and 0, respectively). The blastomeres obtained by biopsy in groups I and II were equally fixed (90% vs. 90%, respectively) and shown in fluorescence in situ hybridization (79% vs. 80%, respectively). Conclusion(s): Compared with the conventional method, the simplified technique is more efficient and equally efficacious for blastomere biopsy in preimplantation genetic diagnosis.

KW - Blastomere biopsy

KW - Fluorescence in situ hybridization

KW - Preimplantation genetic diagnosis

UR - http://www.scopus.com/inward/record.url?scp=0031921221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031921221&partnerID=8YFLogxK

M3 - Article

C2 - 9531901

AN - SCOPUS:0031921221

VL - 69

SP - 569

EP - 575

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 3 SUPPL. 2

ER -