The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos

Shee Uan Chen, Kuang Han Chao, Ming Yih Wu, Chin Der Chen, Hong Nerng Ho, Yu Shih Yang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy. Design: A preclinical, prospective, randomized, in vitro experiment. Setting: The reproductive unit of a university teaching hospital. Patient(s): Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF. Intervention(s): At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously. Main Outcome Measure(s): Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated. Result(s): The mean time (±SD) for biopsy of each embryo in group I (435 ± 137 seconds) was significantly longer than that in group II (126 ± 32 seconds). The success rates for obtaining an intact blastomere were not different between group I (93%) and group II (97%). The growth capacity to the blastocyst stage was similar among the three groups (34%, 37%, and 38%, respectively). However, the ZP-drilled and biopsied embryos of groups I and II had higher percentages of hatching (34% and 37%, respectively) and complete hatching (17% and 20%, respectively) than did those of group III (10% and 0, respectively). The blastomeres obtained by biopsy in groups I and II were equally fixed (90% vs. 90%, respectively) and shown in fluorescence in situ hybridization (79% vs. 80%, respectively). Conclusion(s): Compared with the conventional method, the simplified technique is more efficient and equally efficacious for blastomere biopsy in preimplantation genetic diagnosis.

Original languageEnglish
Pages (from-to)569-575
Number of pages7
JournalFertility and Sterility
Volume69
Issue number3 SUPPL. 2
Publication statusPublished - Dec 1 1998
Externally publishedYes

Fingerprint

Blastomeres
Embryonic Structures
Biopsy
Fluorescence In Situ Hybridization
Zona Pellucida
Preimplantation Diagnosis
Needle Biopsy
Blastocyst
Growth
Teaching Hospitals
Outcome Assessment (Health Care)
Control Groups

Keywords

  • Blastomere biopsy
  • Fluorescence in situ hybridization
  • Preimplantation genetic diagnosis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos. / Chen, Shee Uan; Chao, Kuang Han; Wu, Ming Yih; Chen, Chin Der; Ho, Hong Nerng; Yang, Yu Shih.

In: Fertility and Sterility, Vol. 69, No. 3 SUPPL. 2, 01.12.1998, p. 569-575.

Research output: Contribution to journalArticle

Chen, Shee Uan ; Chao, Kuang Han ; Wu, Ming Yih ; Chen, Chin Der ; Ho, Hong Nerng ; Yang, Yu Shih. / The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos. In: Fertility and Sterility. 1998 ; Vol. 69, No. 3 SUPPL. 2. pp. 569-575.
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AU - Chen, Chin Der

AU - Ho, Hong Nerng

AU - Yang, Yu Shih

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