Abstract
Objectives. Persistence of high-risk types of human papillomavirus (HPV) in cervical scrapes is responsible for the development, maintenance, and progression of squamous intraepithelial lesions (SILs). Previous studies of viral load and histologic severity have ended with controversial results. This study evaluated the relationships of HPV viral load with size and histologic severity of cervical lesions, which has not been reported previously. Methods. By using Hybrid Capture II, DNA level of high-risk HPVs was determined in cervical scrapes and correlated with lesion size and histologic confirmation for 73 women referred for colposcopy. The lesion size was classified as nonvisible (n=12), small (≤2/5 of the 12× colposcopic visual filed, n=36), and large (>2/5 of the 12× field, n=25) lesions. The final disease status was categorized as high-grade SIL (HSIL)/squamous cell carcinoma (SCC) (designated HSIL+) (n=32), low-grade SIL (LSIL) (n=19), and no detectable SIL (n=22). Results. A distinct upward trend of high-risk HPV DNA levels paralleled increasing size and histologic severity of cervical lesions (P=0.003 and 0.001, respectively). With respect to relative risk, women who had high viral load of HPV were at significantly greater risk for large lesion size (odds ratio [OR]=5.3, 95% confidence interval [CI]=1.1-24.9) and HSIL+ (OR=35.0, 95% CI=4.2-294.5). Of particular note, the risk of developing large lesion size and HSIL+ significantly increased with increasing viral load of HPV (P values for trend test were 0.008 and 0.0004, respectively). In contrast, there were no significant associations of trend in viral load with risk in small lesion size and LSIL. Conclusions. The present study revealed that the effect of HPV infection on SIL development is highly influenced by high viral load and highlighted a potential application of viral load testing in predicting the size and severity of lesions of the uterine cervix.
| Original language | English |
|---|---|
| Pages (from-to) | 95-99 |
| Number of pages | 5 |
| Journal | Gynecologic Oncology |
| Volume | 83 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Human papillomavirus
- Hybrid capture II
- Lesion size
- Squamous intraepithelial lesion
- Viral load
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Oncology
Cite this
The significance of human papillomavirus viral load in prediction of histologic severity and size of squamous intraepithelial lesions of uterine cervix. / Sun, Chien An; Lai, Hung Cheng; Chang, Cheng Chang; Neih, Sin; Yu, Cheng Ping; Chu, Tang Yuan.
In: Gynecologic Oncology, Vol. 83, No. 1, 2001, p. 95-99.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The significance of human papillomavirus viral load in prediction of histologic severity and size of squamous intraepithelial lesions of uterine cervix
AU - Sun, Chien An
AU - Lai, Hung Cheng
AU - Chang, Cheng Chang
AU - Neih, Sin
AU - Yu, Cheng Ping
AU - Chu, Tang Yuan
PY - 2001
Y1 - 2001
N2 - Objectives. Persistence of high-risk types of human papillomavirus (HPV) in cervical scrapes is responsible for the development, maintenance, and progression of squamous intraepithelial lesions (SILs). Previous studies of viral load and histologic severity have ended with controversial results. This study evaluated the relationships of HPV viral load with size and histologic severity of cervical lesions, which has not been reported previously. Methods. By using Hybrid Capture II, DNA level of high-risk HPVs was determined in cervical scrapes and correlated with lesion size and histologic confirmation for 73 women referred for colposcopy. The lesion size was classified as nonvisible (n=12), small (≤2/5 of the 12× colposcopic visual filed, n=36), and large (>2/5 of the 12× field, n=25) lesions. The final disease status was categorized as high-grade SIL (HSIL)/squamous cell carcinoma (SCC) (designated HSIL+) (n=32), low-grade SIL (LSIL) (n=19), and no detectable SIL (n=22). Results. A distinct upward trend of high-risk HPV DNA levels paralleled increasing size and histologic severity of cervical lesions (P=0.003 and 0.001, respectively). With respect to relative risk, women who had high viral load of HPV were at significantly greater risk for large lesion size (odds ratio [OR]=5.3, 95% confidence interval [CI]=1.1-24.9) and HSIL+ (OR=35.0, 95% CI=4.2-294.5). Of particular note, the risk of developing large lesion size and HSIL+ significantly increased with increasing viral load of HPV (P values for trend test were 0.008 and 0.0004, respectively). In contrast, there were no significant associations of trend in viral load with risk in small lesion size and LSIL. Conclusions. The present study revealed that the effect of HPV infection on SIL development is highly influenced by high viral load and highlighted a potential application of viral load testing in predicting the size and severity of lesions of the uterine cervix.
AB - Objectives. Persistence of high-risk types of human papillomavirus (HPV) in cervical scrapes is responsible for the development, maintenance, and progression of squamous intraepithelial lesions (SILs). Previous studies of viral load and histologic severity have ended with controversial results. This study evaluated the relationships of HPV viral load with size and histologic severity of cervical lesions, which has not been reported previously. Methods. By using Hybrid Capture II, DNA level of high-risk HPVs was determined in cervical scrapes and correlated with lesion size and histologic confirmation for 73 women referred for colposcopy. The lesion size was classified as nonvisible (n=12), small (≤2/5 of the 12× colposcopic visual filed, n=36), and large (>2/5 of the 12× field, n=25) lesions. The final disease status was categorized as high-grade SIL (HSIL)/squamous cell carcinoma (SCC) (designated HSIL+) (n=32), low-grade SIL (LSIL) (n=19), and no detectable SIL (n=22). Results. A distinct upward trend of high-risk HPV DNA levels paralleled increasing size and histologic severity of cervical lesions (P=0.003 and 0.001, respectively). With respect to relative risk, women who had high viral load of HPV were at significantly greater risk for large lesion size (odds ratio [OR]=5.3, 95% confidence interval [CI]=1.1-24.9) and HSIL+ (OR=35.0, 95% CI=4.2-294.5). Of particular note, the risk of developing large lesion size and HSIL+ significantly increased with increasing viral load of HPV (P values for trend test were 0.008 and 0.0004, respectively). In contrast, there were no significant associations of trend in viral load with risk in small lesion size and LSIL. Conclusions. The present study revealed that the effect of HPV infection on SIL development is highly influenced by high viral load and highlighted a potential application of viral load testing in predicting the size and severity of lesions of the uterine cervix.
KW - Human papillomavirus
KW - Hybrid capture II
KW - Lesion size
KW - Squamous intraepithelial lesion
KW - Viral load
UR - http://www.scopus.com/inward/record.url?scp=0034796235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034796235&partnerID=8YFLogxK
U2 - 10.1006/gyno.2001.6336
DO - 10.1006/gyno.2001.6336
M3 - Article
C2 - 11585419
AN - SCOPUS:0034796235
VL - 83
SP - 95
EP - 99
JO - Gynecologic Oncology
JF - Gynecologic Oncology
SN - 0090-8258
IS - 1
ER -