Abstract

The accumulation of reactive oxygen species (ROS) have implicated the pathogenesis of several human diseases including neurodegenerative disorders, stroke, and traumatic brain injury, hence protecting neurons against ROS is very important. In this study, we focused on sigma-1 receptor (Sig-1R), a chaperone at endoplasmic reticulum, and investigated its protective functions. Using hydrogen peroxide (H2O2)-induced ROS accumulation model, we verified that apoptosis-signaling pathways were elicited by H2O2 treatment. However, the Sig-1R agonists, dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEAS), reduced the activation of apoptotic pathways significantly. By performing protein-protein interaction assays and shRNA knockdown of Sig-1R, we identified the brain Zinc finger protein 179 (Znf179) as a downstream target of Sig-1R regulation. The neuroprotective effect of Znf179 overexpression was similar to that of DHEAS treatment, and likely mediated by affecting the levels of antioxidant enzymes. We also quantified the levels of peroxiredoxin 3 (Prx3) and superoxide dismutase 2 (SOD2) in the hippocampi of wild-type and Znf179 knockout mice, and found both enzymes to be reduced in the knockout versus the wild-type mice. In summary, these results reveal that Znf179 plays a novel role in neuroprotection, and Sig-1R agonists may be therapeutic candidates to prevent ROS-induced damage in neurodegenerative and neurotraumatic diseases.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalNeuropharmacology
Volume105
DOIs
Publication statusPublished - Jun 1 2016

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Zinc Fingers
Hydrogen Peroxide
Reactive Oxygen Species
Wounds and Injuries
Dehydroepiandrosterone Sulfate
Proteins
Neurodegenerative Diseases
Peroxiredoxin III
Dehydroepiandrosterone
Neuroprotective Agents
Enzymes
Knockout Mice
Endoplasmic Reticulum
Small Interfering RNA
Hippocampus
Antioxidants
Stroke
sigma-1 receptor
Apoptosis
Neurons

Keywords

  • DHEA/DHEAS
  • Hydrogen peroxide
  • Reactive oxygen species
  • Sig-1R
  • Znf179

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

The sigma-1 receptor-zinc finger protein 179 pathway protects against hydrogen peroxide-induced cell injury. / Su, Tzu Chieh; Lin, Shu Hui; Lee, Pin Tse; Yeh, Shiu Hwa; Hsieh, Tsung Hsun; Chou, Szu Yi; Su, Tsung Ping; Hung, Jan Jong; Chang, Wen Chang; Lee, Yi Chao; Chuang, Jian Ying.

In: Neuropharmacology, Vol. 105, 01.06.2016, p. 1-9.

Research output: Contribution to journalArticle

Su, Tzu Chieh ; Lin, Shu Hui ; Lee, Pin Tse ; Yeh, Shiu Hwa ; Hsieh, Tsung Hsun ; Chou, Szu Yi ; Su, Tsung Ping ; Hung, Jan Jong ; Chang, Wen Chang ; Lee, Yi Chao ; Chuang, Jian Ying. / The sigma-1 receptor-zinc finger protein 179 pathway protects against hydrogen peroxide-induced cell injury. In: Neuropharmacology. 2016 ; Vol. 105. pp. 1-9.
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AU - Hsieh, Tsung Hsun

AU - Chou, Szu Yi

AU - Su, Tsung Ping

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AB - The accumulation of reactive oxygen species (ROS) have implicated the pathogenesis of several human diseases including neurodegenerative disorders, stroke, and traumatic brain injury, hence protecting neurons against ROS is very important. In this study, we focused on sigma-1 receptor (Sig-1R), a chaperone at endoplasmic reticulum, and investigated its protective functions. Using hydrogen peroxide (H2O2)-induced ROS accumulation model, we verified that apoptosis-signaling pathways were elicited by H2O2 treatment. However, the Sig-1R agonists, dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEAS), reduced the activation of apoptotic pathways significantly. By performing protein-protein interaction assays and shRNA knockdown of Sig-1R, we identified the brain Zinc finger protein 179 (Znf179) as a downstream target of Sig-1R regulation. The neuroprotective effect of Znf179 overexpression was similar to that of DHEAS treatment, and likely mediated by affecting the levels of antioxidant enzymes. We also quantified the levels of peroxiredoxin 3 (Prx3) and superoxide dismutase 2 (SOD2) in the hippocampi of wild-type and Znf179 knockout mice, and found both enzymes to be reduced in the knockout versus the wild-type mice. In summary, these results reveal that Znf179 plays a novel role in neuroprotection, and Sig-1R agonists may be therapeutic candidates to prevent ROS-induced damage in neurodegenerative and neurotraumatic diseases.

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