The sequential changes of the virion polypeptides of CV HB1 virus (Birnaviridae) during serial undiluted passaging

Chu Fang Lo, Shau Chi Chi, Nai Yueh Tien, Pei Jung Yu, Chih Ming Chou, Shiu Nan Chen, Chung Hsiung Wang, Guang Hsiung Kou

Research output: Contribution to journalArticle

Abstract

Clam virus (CV HB-1) isolated from hard clam Meretrix lusoria, typed as infectious pancreatic necrosis virus (IPNV) Ab serotype, was employed for the present study. The structural polypeptides of purified virus generated by serial undiluted and diluted passaging were analyzed using sodiumdodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results indicated that in VP2, the virus particles of serial undiluted passaging had viral polypeptides smaller than those of serial diluted passaging. Furthermore, a polypeptide with a molecular mass of 105 kDa was found in the serial undiluted virus preparation. Further analysis by monoclonal antibodies showed that VP105 might be a polyprotein produced by genomic RNA segment A. To investigate the sequential changes of viral polypeptides of the virus preparation at high multiplicity infection, the viral polypeptides at each undiluted passage were analyzed by SDS-PAGE followed by immunoblotting. The results indicated that VP105 was present by undiluted passage 7, while the smaller VP2 was present by undiluted passage 10. The amount of smaller VP2 and VP105 increased as the number of passages increased.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalAquaculture
Volume132
Issue number1-2
DOIs
Publication statusPublished - Apr 15 1995
Externally publishedYes

Fingerprint

Birnaviridae
virion
polypeptides
virus
viruses
clams
polyacrylamide gel electrophoresis
electrokinesis
sulfates
gel
sulfate
Infectious pancreatic necrosis virus
virus particle
immunoblotting
antibody
RNA
monoclonal antibodies
genomics
serotypes
molecular weight

Keywords

  • Birnaviridae
  • Meretrix lusoria
  • Virion polypeptides, sequential changes
  • Viruses, undiluted passaging

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The sequential changes of the virion polypeptides of CV HB1 virus (Birnaviridae) during serial undiluted passaging. / Lo, Chu Fang; Chi, Shau Chi; Tien, Nai Yueh; Yu, Pei Jung; Chou, Chih Ming; Chen, Shiu Nan; Wang, Chung Hsiung; Kou, Guang Hsiung.

In: Aquaculture, Vol. 132, No. 1-2, 15.04.1995, p. 73-80.

Research output: Contribution to journalArticle

Lo, Chu Fang ; Chi, Shau Chi ; Tien, Nai Yueh ; Yu, Pei Jung ; Chou, Chih Ming ; Chen, Shiu Nan ; Wang, Chung Hsiung ; Kou, Guang Hsiung. / The sequential changes of the virion polypeptides of CV HB1 virus (Birnaviridae) during serial undiluted passaging. In: Aquaculture. 1995 ; Vol. 132, No. 1-2. pp. 73-80.
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AB - Clam virus (CV HB-1) isolated from hard clam Meretrix lusoria, typed as infectious pancreatic necrosis virus (IPNV) Ab serotype, was employed for the present study. The structural polypeptides of purified virus generated by serial undiluted and diluted passaging were analyzed using sodiumdodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results indicated that in VP2, the virus particles of serial undiluted passaging had viral polypeptides smaller than those of serial diluted passaging. Furthermore, a polypeptide with a molecular mass of 105 kDa was found in the serial undiluted virus preparation. Further analysis by monoclonal antibodies showed that VP105 might be a polyprotein produced by genomic RNA segment A. To investigate the sequential changes of viral polypeptides of the virus preparation at high multiplicity infection, the viral polypeptides at each undiluted passage were analyzed by SDS-PAGE followed by immunoblotting. The results indicated that VP105 was present by undiluted passage 7, while the smaller VP2 was present by undiluted passage 10. The amount of smaller VP2 and VP105 increased as the number of passages increased.

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