The roles of RGD and grooved topography in the adhesion, morphology, and differentiation of C2C12 skeletal myoblasts

Peng Yuan Wang, Helmut Thissen, Wei Bor Tsai

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Both chemical and topographic cues are crucial for the development of skeletal muscle. In this study, the relative roles of both signals in regard to cell adhesion, morphology, and differentiation of C2C12 skeletal myoblasts were investigated. Grooved polystyrene substrates containing grooves with approximately 900nm in width with 600nm ridge spans and 665nm in depth were conjugated with the cell adhesion peptide arginine-glycine-aspartic acid (RGD). RGD conjugation significantly enhanced the adhesion, growth and differentiation of C2C12 cells. On the other hand, anisotropic topography primarily directed the direction and alignment of myoblasts and myotubes. The results in this study provide information regarding the relative roles of chemical and topographic cues in musculoskeletal myogenesis, and are of interest to applications in muscle tissue engineering.

Original languageEnglish
Pages (from-to)2104-2115
Number of pages12
JournalBiotechnology and Bioengineering
Volume109
Issue number8
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

Fingerprint

Skeletal Myoblasts
Cell adhesion
Cell Adhesion
Topography
Cues
Muscle
Cell Differentiation
Adhesion
Arginine
Muscle Development
Myoblasts
Polystyrenes
Skeletal Muscle Fibers
Tissue Engineering
Tissue engineering
Aspartic Acid
Glycine
Peptides
Amino acids
Skeletal Muscle

Keywords

  • Alignment
  • Grooves
  • Myotubes
  • RGD
  • Skeletal myoblasts

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

The roles of RGD and grooved topography in the adhesion, morphology, and differentiation of C2C12 skeletal myoblasts. / Wang, Peng Yuan; Thissen, Helmut; Tsai, Wei Bor.

In: Biotechnology and Bioengineering, Vol. 109, No. 8, 08.2012, p. 2104-2115.

Research output: Contribution to journalArticle

Wang, Peng Yuan ; Thissen, Helmut ; Tsai, Wei Bor. / The roles of RGD and grooved topography in the adhesion, morphology, and differentiation of C2C12 skeletal myoblasts. In: Biotechnology and Bioengineering. 2012 ; Vol. 109, No. 8. pp. 2104-2115.
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