The role of peroxiredoxin II in radiation-resistant MCF-7 breast cancer cells

Tieli Wang, Daniel Tamae, Thomas LeBon, John E. Shively, Yun Yen, Jian Jian Li

Research output: Contribution to journalArticle

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Abstract

Although several signaling pathways have been suggested to be involved in the cellular response to ionizing radiation, the molecular basis of tumor resistance to radiation remains elusive. We have developed a unique model system based upon the MCF-7 human breast cancer cell line that became resistant to radiation treatment (MCF+FIR30) after exposure to chronic ionizing radiation. By proteomics analysis, we found that peroxiredoxin II (PrxII), a member of a family of peroxidases, is up-regulated in the radiation-derived MCF+FIR3 cells but not in the MCF+FIS4 cells that are relatively sensitive to radiation. Both MCF+FIR3 and MCF+FIS4 cell lines are from MCF+FIR30 populations. Furthermore, the resistance to ionizing radiation can be partially reversed by silencing the expression of PrxII by PrxII/small interfering RNA treatment of MCF+FIR3 resistant cells, suggesting that PrxII is not the sole factor responsible for the resistant phenotype. The relevance of this mechanism was further confirmed by the increased radioresistance in PrxII-overexpressing MCF+FIS4 cells when compared with vector control cells. The upregulation of the PrxII protein in radioresistant cancer cells suggested that human peroxiredoxin plays an important role in eliminating the generation of reactive oxygen species by ionizing radiation. The present finding, together with the observation that PrxII is also up-regulated in response to ionizing radiation in other cell systems, strengthens the hypothesis that the PrxII antioxidant protein is involved in the cellular response to ionizing radiation and functions to reduce the intracellular reactive oxygen species levels, resulting in increased resistance of breast cancer cells to ionizing radiation.

Original languageEnglish
Pages (from-to)10338-10346
Number of pages9
JournalCancer Research
Volume65
Issue number22
DOIs
Publication statusPublished - Nov 15 2005
Externally publishedYes

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Peroxiredoxins
Ionizing Radiation
Radiation
Breast Neoplasms
Reactive Oxygen Species
Peroxidases
Cell Line
Proteomics
Small Interfering RNA
Neoplasms
Proteins
Up-Regulation
Antioxidants
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The role of peroxiredoxin II in radiation-resistant MCF-7 breast cancer cells. / Wang, Tieli; Tamae, Daniel; LeBon, Thomas; Shively, John E.; Yen, Yun; Li, Jian Jian.

In: Cancer Research, Vol. 65, No. 22, 15.11.2005, p. 10338-10346.

Research output: Contribution to journalArticle

Wang, Tieli ; Tamae, Daniel ; LeBon, Thomas ; Shively, John E. ; Yen, Yun ; Li, Jian Jian. / The role of peroxiredoxin II in radiation-resistant MCF-7 breast cancer cells. In: Cancer Research. 2005 ; Vol. 65, No. 22. pp. 10338-10346.
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