The role of aldehyde dehydrogenase and Hsp70 in suppression of white spot syndrome virus replication at high temperature

Ying Ru Lin, Hsiao Chun Hung, Jiann Horng Leu, Hao Ching Wang, Guang Hsiung Kou, Chu Fang Lo

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

High temperature (32 to 33°C) has been shown to reduce mortality in white spot syndrome virus (WSSV)-infected shrimps, but the mechanism still remains unclear. Here we show that in WSSV-infected shrimps cultured at 32°C, transcriptional levels of representative immediate-early, early, and late genes were initially higher than those at 25°C. However, neither the IE1 nor VP28 protein was detected at 32°C, suggesting that high temperature might inhibit WSSV protein synthesis. Two-dimensional gel electrophoresis analysis revealed two proteins, NAD-dependent aldehyde dehydrogenase (ALDH) and the proteasome alpha 4 subunit (proteasome α4), that were markedly upregulated in WSSV-infected shrimps at 32°C. Reverse transcription-PCR (RT-PCR) analysis of members of the heat shock protein family also showed that hsp70 was upregulated at 32°C. When aldh, proteasome α4, and hsp70 were knocked down by double-stranded RNA interference and shrimps were challenged with WSSV, the aldh and hsp70 knockdown shrimps became severely infected at 32°C, while the proteasome α4 knockdown shrimps remained uninfected. Our results therefore suggest that ALDH and Hsp70 both play an important role in the inhibition of WSSV replication at high temperature.

Original languageEnglish
Pages (from-to)3517-3525
Number of pages9
JournalJournal of Virology
Volume85
Issue number7
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Fingerprint

White spot syndrome virus 1
aldehyde dehydrogenase
White spot syndrome virus
Aldehyde Dehydrogenase
Virus Replication
virus replication
shrimp
Temperature
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
temperature
Proteins
Double-Stranded RNA
two-dimensional gel electrophoresis
double-stranded RNA
Electrophoresis, Gel, Two-Dimensional
Heat-Shock Proteins
RNA Interference
RNA interference
heat shock proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The role of aldehyde dehydrogenase and Hsp70 in suppression of white spot syndrome virus replication at high temperature. / Lin, Ying Ru; Hung, Hsiao Chun; Leu, Jiann Horng; Wang, Hao Ching; Kou, Guang Hsiung; Lo, Chu Fang.

In: Journal of Virology, Vol. 85, No. 7, 04.2011, p. 3517-3525.

Research output: Contribution to journalArticle

Lin, Ying Ru ; Hung, Hsiao Chun ; Leu, Jiann Horng ; Wang, Hao Ching ; Kou, Guang Hsiung ; Lo, Chu Fang. / The role of aldehyde dehydrogenase and Hsp70 in suppression of white spot syndrome virus replication at high temperature. In: Journal of Virology. 2011 ; Vol. 85, No. 7. pp. 3517-3525.
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