The risk of hydroquinone and sunscreen over-absorption via photodamaged skin is not greater in senescent skin as compared to young skin: Nude mouse as an animal model

Chi Feng Hung, Wei Yu Chen, Ibrahim A. Aljuffali, Hui Chi Shih, Jia You Fang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Intrinsic aging and photoaging modify skin structure and components, which subsequently change percutaneous absorption of topically applied permeants. The purpose of this study was to systematically evaluate drug/sunscreen permeation via young and senescent skin irradiated by ultraviolet (UV) light. Both young and senescent nude mice were subjected to UVA (10 J/cm2) and/or UVB radiation (175 mJ/cm2). Physiological parameters, immunohistology, and immunoblotting were employed to examine the aged skin. Hydroquinone and sunscreen permeation was determined by in vitro Franz cell. In vivo skin absorption was documented using a hydrophilic dye, rhodamine 123 (log P = -0.4), as a permeant. UVA exposure induced cyclooxygenase (COX)-2 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) upregulation. Epidermal tight junction (TJ) were degraded by UVA. UVB increased transepidermal water loss (TEWL) from 13 to 24 g/m2/h. Hyperplasia and inflammation, but not loss of TJ, were also observed in UVB-treated skin. UVA + UVB- and UVA-irradiated skin demonstrated similar changes in histology and biomarkers. UVA + UVB or UVA exposure increased hydroquinone flux five-fold. A negligible alteration of hydroquinone permeation was shown with UVB exposure. Hydroquinone exhibited a lower penetration through senescent skin than young skin. Both UVA and UVB produced enhancement of oxybenzone flux and skin uptake. However, the amount of increase was less than that of hydroquinone delivery. Photoaging did not augment skin absorption of sunscreens with higher lipophilicity, including avobenzone and ZnO. Exposure to UVA generally increased follicular entrance of these permeants, which showed two- to three-fold greater follicular uptake compared to the untreated group. Photoaging had less impact on drug/sunscreen absorption with more lipophilic permeants. Percutaneous absorption did not increase in skin subjected to both intrinsic and extrinsic aging.

Original languageEnglish
Pages (from-to)135-145
Number of pages11
JournalInternational Journal of Pharmaceutics
Volume471
Issue number1-2
DOIs
Publication statusPublished - Aug 25 2014

Fingerprint

Sunscreening Agents
Nude Mice
Animal Models
Skin Absorption
Skin
Tight Junctions
Rhodamine 123
Skin Aging
DNA Nucleotidylexotransferase
hydroquinone
Cyclooxygenase 2
Ultraviolet Rays
Immunoblotting
Pharmaceutical Preparations
Hyperplasia
Histology
Coloring Agents
Up-Regulation
Biomarkers
Radiation

Keywords

  • Hydroquinone
  • Intrinsic aging
  • Percutaneous absorption
  • Photoaging
  • Sunscreen
  • Tight junction

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine(all)

Cite this

The risk of hydroquinone and sunscreen over-absorption via photodamaged skin is not greater in senescent skin as compared to young skin : Nude mouse as an animal model. / Hung, Chi Feng; Chen, Wei Yu; Aljuffali, Ibrahim A.; Shih, Hui Chi; Fang, Jia You.

In: International Journal of Pharmaceutics, Vol. 471, No. 1-2, 25.08.2014, p. 135-145.

Research output: Contribution to journalArticle

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abstract = "Intrinsic aging and photoaging modify skin structure and components, which subsequently change percutaneous absorption of topically applied permeants. The purpose of this study was to systematically evaluate drug/sunscreen permeation via young and senescent skin irradiated by ultraviolet (UV) light. Both young and senescent nude mice were subjected to UVA (10 J/cm2) and/or UVB radiation (175 mJ/cm2). Physiological parameters, immunohistology, and immunoblotting were employed to examine the aged skin. Hydroquinone and sunscreen permeation was determined by in vitro Franz cell. In vivo skin absorption was documented using a hydrophilic dye, rhodamine 123 (log P = -0.4), as a permeant. UVA exposure induced cyclooxygenase (COX)-2 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) upregulation. Epidermal tight junction (TJ) were degraded by UVA. UVB increased transepidermal water loss (TEWL) from 13 to 24 g/m2/h. Hyperplasia and inflammation, but not loss of TJ, were also observed in UVB-treated skin. UVA + UVB- and UVA-irradiated skin demonstrated similar changes in histology and biomarkers. UVA + UVB or UVA exposure increased hydroquinone flux five-fold. A negligible alteration of hydroquinone permeation was shown with UVB exposure. Hydroquinone exhibited a lower penetration through senescent skin than young skin. Both UVA and UVB produced enhancement of oxybenzone flux and skin uptake. However, the amount of increase was less than that of hydroquinone delivery. Photoaging did not augment skin absorption of sunscreens with higher lipophilicity, including avobenzone and ZnO. Exposure to UVA generally increased follicular entrance of these permeants, which showed two- to three-fold greater follicular uptake compared to the untreated group. Photoaging had less impact on drug/sunscreen absorption with more lipophilic permeants. Percutaneous absorption did not increase in skin subjected to both intrinsic and extrinsic aging.",
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