The regulation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumor promotion by estradiol in female A/J mice

Rong Jane Chen, Chu Yung Chang, Louis W. Chang, Shih He Siao, Yuan Soon Ho, Chih Hsiung Wu, Ning Ping Foo, Pinpin Lin, Ying Jan Wang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Epidemiological studies indicate that women are at a higher risk developing lung cancer than men are. It is suggested that estrogen is one of the most important factors in lung cancer development in females. Additionally, cigarette smoke, and environmental pollutants, such as 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD), may play salient roles in female lung carcinogenesis. However, the mechanisms responsible for the interaction of these factors in the promotion of lung cancer are still poorly understood. The present study was designed to explore two ideas: first, the synergistic lung tumorigenic effects of 4-(methylnitrosamino)-1-(3-pyridyl)-butanol (NNK) combined with TCDD, 17β-estradiol (E2) or both through a long-term treatment experiment, and second, to identify early changes in the inflammatory and signaling pathways through short-term treatment experiments. The results indicate that A/J mice given E2 had strong effects in potentiating NNK-induced activation of MAPK signaling, NFkB, and COX-2 expression. In the long-term exposure model, E2 had a strong tumor promoting effect, whereas TCDD antagonized this effect in A/J mice. We conclude that treatment with NNK combined with either E2 or TCDD induces lung carcinogenesis and the promotion effects could be correlated with lung inflammation. E2 was shown to potentiate NNK-induced inflammation, cell proliferation, thereby leading to lung tumorigenesis.

Original languageEnglish
Article numbere93152
JournalPLoS One
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 28 2014

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tetrachlorodibenzo-p-dioxin
lung neoplasms
estradiol
Tumors
Estradiol
lungs
Lung Neoplasms
Lung
Carcinogenesis
carcinogenesis
mice
Neoplasms
Environmental Pollutants
Butanols
inflammation
exposure models
Cell proliferation
Smoke
Tobacco Products
cigarettes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The regulation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumor promotion by estradiol in female A/J mice. / Chen, Rong Jane; Chang, Chu Yung; Chang, Louis W.; Siao, Shih He; Ho, Yuan Soon; Wu, Chih Hsiung; Foo, Ning Ping; Lin, Pinpin; Wang, Ying Jan.

In: PLoS One, Vol. 9, No. 3, e93152, 28.03.2014.

Research output: Contribution to journalArticle

Chen, Rong Jane ; Chang, Chu Yung ; Chang, Louis W. ; Siao, Shih He ; Ho, Yuan Soon ; Wu, Chih Hsiung ; Foo, Ning Ping ; Lin, Pinpin ; Wang, Ying Jan. / The regulation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumor promotion by estradiol in female A/J mice. In: PLoS One. 2014 ; Vol. 9, No. 3.
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