The rDNA sequence analysis of three Dendrobium species

Kur-Ta Cheng, Shu Fang Lo, Chun Yue Lee, Chung Chuan Chen, Hsin Sheng Tsay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Positive molecular identification of medicinal plants is necessary to their misuse. Dendrobii Caulis is a famous Chinese herbal medicine traditionally used in ophthalmology. However, the identification of the species based on morphology is difficult. In this study, three Dendrobium species were collected and differentiated by the sequences of partial rDNA (ribosomal DNA) sections which contain the ITS 1, 5.8S, and ITS 2 regions. The Dendrobium species used in this reseach included Dendrobium tosaense, Dendrobium officinale, and Dendrobium moniliforme. The length of PCR products are 632 nt for D. tosaense, 627 nt for D. officinale, and 628 nt for D. moniliform, respectively. The similarities of the rDNA region between the species pairs shown range from 91% to 95%. The results we present here may be helpful in identifying other species of Dendrobium.

Original languageEnglish
Pages (from-to)367-369
Number of pages3
JournalJournal of Food and Drug Analysis
Volume12
Issue number4
Publication statusPublished - Dec 2004

Fingerprint

Dendrobium
Ribosomal DNA
DNA Sequence Analysis
ribosomal DNA
Dendrobium officinale
sequence analysis
nucleotide sequences
ophthalmology
herbal medicines
medicinal plants
Herbal Medicine
Ophthalmology
Medicinal Plants
Polymerase Chain Reaction

Keywords

  • Dendrobium
  • ITS
  • Phylogeny
  • rDNA

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Cheng, K-T., Lo, S. F., Lee, C. Y., Chen, C. C., & Tsay, H. S. (2004). The rDNA sequence analysis of three Dendrobium species. Journal of Food and Drug Analysis, 12(4), 367-369.

The rDNA sequence analysis of three Dendrobium species. / Cheng, Kur-Ta; Lo, Shu Fang; Lee, Chun Yue; Chen, Chung Chuan; Tsay, Hsin Sheng.

In: Journal of Food and Drug Analysis, Vol. 12, No. 4, 12.2004, p. 367-369.

Research output: Contribution to journalArticle

Cheng, K-T, Lo, SF, Lee, CY, Chen, CC & Tsay, HS 2004, 'The rDNA sequence analysis of three Dendrobium species', Journal of Food and Drug Analysis, vol. 12, no. 4, pp. 367-369.
Cheng, Kur-Ta ; Lo, Shu Fang ; Lee, Chun Yue ; Chen, Chung Chuan ; Tsay, Hsin Sheng. / The rDNA sequence analysis of three Dendrobium species. In: Journal of Food and Drug Analysis. 2004 ; Vol. 12, No. 4. pp. 367-369.
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