The rDNA sequence analysis of three Dendrobium species

Kur-Ta Cheng, Shu Fang Lo, Chun Yue Lee, Chung Chuan Chen, Hsin Sheng Tsay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Positive molecular identification of medicinal plants is necessary to their misuse. Dendrobii Caulis is a famous Chinese herbal medicine traditionally used in ophthalmology. However, the identification of the species based on morphology is difficult. In this study, three Dendrobium species were collected and differentiated by the sequences of partial rDNA (ribosomal DNA) sections which contain the ITS 1, 5.8S, and ITS 2 regions. The Dendrobium species used in this reseach included Dendrobium tosaense, Dendrobium officinale, and Dendrobium moniliforme. The length of PCR products are 632 nt for D. tosaense, 627 nt for D. officinale, and 628 nt for D. moniliform, respectively. The similarities of the rDNA region between the species pairs shown range from 91% to 95%. The results we present here may be helpful in identifying other species of Dendrobium.

Original languageEnglish
Pages (from-to)367-369
Number of pages3
JournalJournal of Food and Drug Analysis
Volume12
Issue number4
Publication statusPublished - Dec 2004

Keywords

  • Dendrobium
  • ITS
  • Phylogeny
  • rDNA

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Fingerprint Dive into the research topics of 'The rDNA sequence analysis of three Dendrobium species'. Together they form a unique fingerprint.

  • Cite this

    Cheng, K-T., Lo, S. F., Lee, C. Y., Chen, C. C., & Tsay, H. S. (2004). The rDNA sequence analysis of three Dendrobium species. Journal of Food and Drug Analysis, 12(4), 367-369.