The Protective Role of Heme Oxygenase-1 Induction on Testicular Tissues After Testicular Torsion and Detorsion

Stone Yang, Hung Jen Shih, Yung Chiong Chow, Pei Shan Tsai, Tao Yeuan Wang, Paulus S. Wang, Chun Jen Huang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose: Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups. Results: Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin. Conclusions: Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.

Original languageEnglish
Pages (from-to)1928-1933
Number of pages6
JournalJournal of Urology
Volume177
Issue number5
DOIs
Publication statusPublished - May 2007

Fingerprint

Spermatic Cord Torsion
Heme Oxygenase-1
Hemin
Wounds and Injuries
Malondialdehyde
Peroxidase
Testis
Nitric Oxide
Injections
Reperfusion Injury
Sprague Dawley Rats
Control Groups

Keywords

  • heme oxygenase (decyclizing)
  • hemin
  • rats
  • spermatic cord torsion
  • Sprague-Dawley
  • testis

ASJC Scopus subject areas

  • Urology

Cite this

The Protective Role of Heme Oxygenase-1 Induction on Testicular Tissues After Testicular Torsion and Detorsion. / Yang, Stone; Shih, Hung Jen; Chow, Yung Chiong; Tsai, Pei Shan; Wang, Tao Yeuan; Wang, Paulus S.; Huang, Chun Jen.

In: Journal of Urology, Vol. 177, No. 5, 05.2007, p. 1928-1933.

Research output: Contribution to journalArticle

@article{6d0090b63bff44aa8a0cfc1787897277,
title = "The Protective Role of Heme Oxygenase-1 Induction on Testicular Tissues After Testicular Torsion and Detorsion",
abstract = "Purpose: Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups. Results: Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin. Conclusions: Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.",
keywords = "heme oxygenase (decyclizing), hemin, rats, spermatic cord torsion, Sprague-Dawley, testis",
author = "Stone Yang and Shih, {Hung Jen} and Chow, {Yung Chiong} and Tsai, {Pei Shan} and Wang, {Tao Yeuan} and Wang, {Paulus S.} and Huang, {Chun Jen}",
year = "2007",
month = "5",
doi = "10.1016/j.juro.2007.01.015",
language = "English",
volume = "177",
pages = "1928--1933",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - The Protective Role of Heme Oxygenase-1 Induction on Testicular Tissues After Testicular Torsion and Detorsion

AU - Yang, Stone

AU - Shih, Hung Jen

AU - Chow, Yung Chiong

AU - Tsai, Pei Shan

AU - Wang, Tao Yeuan

AU - Wang, Paulus S.

AU - Huang, Chun Jen

PY - 2007/5

Y1 - 2007/5

N2 - Purpose: Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups. Results: Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin. Conclusions: Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.

AB - Purpose: Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups. Results: Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin. Conclusions: Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.

KW - heme oxygenase (decyclizing)

KW - hemin

KW - rats

KW - spermatic cord torsion

KW - Sprague-Dawley

KW - testis

UR - http://www.scopus.com/inward/record.url?scp=34147127724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147127724&partnerID=8YFLogxK

U2 - 10.1016/j.juro.2007.01.015

DO - 10.1016/j.juro.2007.01.015

M3 - Article

C2 - 17437850

AN - SCOPUS:34147127724

VL - 177

SP - 1928

EP - 1933

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 5

ER -