The Protective Role of Heme Oxygenase-1 Induction on Testicular Tissues After Testicular Torsion and Detorsion

Stone Yang, Hung Jen Shih, Yung Chiong Chow, Pei Shan Tsai, Tao Yeuan Wang, Paulus S. Wang, Chun Jen Huang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups. Results: Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin. Conclusions: Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.

Original languageEnglish
Pages (from-to)1928-1933
Number of pages6
JournalJournal of Urology
Volume177
Issue number5
DOIs
Publication statusPublished - May 2007

Keywords

  • heme oxygenase (decyclizing)
  • hemin
  • rats
  • spermatic cord torsion
  • Sprague-Dawley
  • testis

ASJC Scopus subject areas

  • Urology

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