The protease allergen Pen c 13 induces allergic airway inflammation and changes in epithelial barrier integrity and function in a murine model

Jui Chieh Chen, Jiing Guang Chuang, Yu Yi Su, Bor Luen Chiang, You Shuei Lin, Lu Ping Chow

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Fungal allergens are associated with the development of asthma, and some have been characterized as proteases. Here, we established an animal model of allergic airway inflammation in response to continuous exposure to proteolytically active Pen c 13, a major allergen secreted by Penicillium citrinum. In functional analyses, Pen c 13 exposure led to increased airway hyperresponsiveness, significant inflammatory cell infiltration, mucus overproduction, and collagen deposition in the lung, dramatically elevated serum levels of total IgE and Pen c 13-specific IgE and IgG1, and increased production of the Th2 cytokines IL-4, IL-5, and IL-13 by splenocytes stimulated in vitro with Pen c 13. To examine the mechanisms involved in the regulation of allergenicity by Pen c 13, we performed two-dimensional fluorescence difference gel electrophoresis analysis combined with nano-LC-MS/MS, followed by bioinformatics analysis to identify potential targets that associated with allergic inflammation, which suggested that galectin-3 and laminin might be involved in novel pathogenic mechanisms. Finally, we focused on junctional proteins between cells, because, in addition to opening of the epithelial barrier by environmental proteases possibly being the initial step in the development of asthma, these proteins are also associated with actin rearrangement. Taken together, our findings indicate that Pen c 13 exposure causes junctional structure alterations and actin cytoskeletal rearrangements, resulting in increased permeability and airway structural changes. These effects probably change the lung microenvironment and foster the development of allergic sensitization.

Original languageEnglish
Pages (from-to)26667-26679
Number of pages13
JournalJournal of Biological Chemistry
Volume286
Issue number30
DOIs
Publication statusPublished - Jul 29 2011

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Allergens
Immunoglobulin E
Actins
Peptide Hydrolases
Asthma
Two-Dimensional Difference Gel Electrophoresis
Inflammation
Galectin 3
Lung
Interleukin-13
Penicillium
Interleukin-5
Laminin
Mucus
Computational Biology
Interleukin-4
Permeability
Proteins
Collagen
Animal Models

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The protease allergen Pen c 13 induces allergic airway inflammation and changes in epithelial barrier integrity and function in a murine model. / Chen, Jui Chieh; Chuang, Jiing Guang; Su, Yu Yi; Chiang, Bor Luen; Lin, You Shuei; Chow, Lu Ping.

In: Journal of Biological Chemistry, Vol. 286, No. 30, 29.07.2011, p. 26667-26679.

Research output: Contribution to journalArticle

Chen, Jui Chieh ; Chuang, Jiing Guang ; Su, Yu Yi ; Chiang, Bor Luen ; Lin, You Shuei ; Chow, Lu Ping. / The protease allergen Pen c 13 induces allergic airway inflammation and changes in epithelial barrier integrity and function in a murine model. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 30. pp. 26667-26679.
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