The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer

T. H. Chen, S. W. Chang, C. C. Huang, K. L. Wang, K. T. Yeh, C. N. Liu, H. Lee, C. C. Lin, Y. W. Cheng

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Aim: Colorectal cancer (CRC) is the second commonest cause of cancer death in Taiwan. Although numerous genes have been associated with tumorigenesis in colorectal cancer, only a few have been validated and used as biomarkers for predicting clinical outcome. The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients. Method: In total, 195 colorectal cancer patients were enrolled in a single medical centre between 2003 and 2007. APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction. The primary outcome included 5-year overall survival and univariate (Kaplan-Meier) and multivariate (Cox regression) analyses of prognostic factors. Results: The results showed that 66 (33.8%) of 195 tumour tissues contained an APC mutation. The predominant APC gene variations were deletion mutations (50.0%). APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation. In advanced-stage cancer, patients with APC mutation/high miR-21 had poorer overall survival rates than those with APC mutation/low miR-21, APC wild-type/high miR-21 and APC wild-type/low miR-21. Conclusion: In Taiwan, downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation. APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC, especially in patients with advanced disease.

Original languageEnglish
Pages (from-to)1367-1374
Number of pages8
JournalColorectal Disease
Volume15
Issue number11
DOIs
Publication statusPublished - Nov 2013

Fingerprint

APC Genes
Colorectal Neoplasms
Mutation
Taiwan
Gene Expression
Neoplasms
Sequence Deletion
DNA Sequence Analysis
Genes
Reverse Transcription
Cause of Death
Carcinogenesis
Up-Regulation
Down-Regulation
Survival Rate
Biomarkers
Regression Analysis
Polymerase Chain Reaction
Survival

Keywords

  • APC
  • MiR-21
  • Prognostic factor

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Chen, T. H., Chang, S. W., Huang, C. C., Wang, K. L., Yeh, K. T., Liu, C. N., ... Cheng, Y. W. (2013). The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer. Colorectal Disease, 15(11), 1367-1374. https://doi.org/10.1111/codi.12318

The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer. / Chen, T. H.; Chang, S. W.; Huang, C. C.; Wang, K. L.; Yeh, K. T.; Liu, C. N.; Lee, H.; Lin, C. C.; Cheng, Y. W.

In: Colorectal Disease, Vol. 15, No. 11, 11.2013, p. 1367-1374.

Research output: Contribution to journalArticle

Chen, TH, Chang, SW, Huang, CC, Wang, KL, Yeh, KT, Liu, CN, Lee, H, Lin, CC & Cheng, YW 2013, 'The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer', Colorectal Disease, vol. 15, no. 11, pp. 1367-1374. https://doi.org/10.1111/codi.12318
Chen, T. H. ; Chang, S. W. ; Huang, C. C. ; Wang, K. L. ; Yeh, K. T. ; Liu, C. N. ; Lee, H. ; Lin, C. C. ; Cheng, Y. W. / The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer. In: Colorectal Disease. 2013 ; Vol. 15, No. 11. pp. 1367-1374.
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abstract = "Aim: Colorectal cancer (CRC) is the second commonest cause of cancer death in Taiwan. Although numerous genes have been associated with tumorigenesis in colorectal cancer, only a few have been validated and used as biomarkers for predicting clinical outcome. The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients. Method: In total, 195 colorectal cancer patients were enrolled in a single medical centre between 2003 and 2007. APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction. The primary outcome included 5-year overall survival and univariate (Kaplan-Meier) and multivariate (Cox regression) analyses of prognostic factors. Results: The results showed that 66 (33.8{\%}) of 195 tumour tissues contained an APC mutation. The predominant APC gene variations were deletion mutations (50.0{\%}). APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation. In advanced-stage cancer, patients with APC mutation/high miR-21 had poorer overall survival rates than those with APC mutation/low miR-21, APC wild-type/high miR-21 and APC wild-type/low miR-21. Conclusion: In Taiwan, downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation. APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC, especially in patients with advanced disease.",
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N2 - Aim: Colorectal cancer (CRC) is the second commonest cause of cancer death in Taiwan. Although numerous genes have been associated with tumorigenesis in colorectal cancer, only a few have been validated and used as biomarkers for predicting clinical outcome. The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients. Method: In total, 195 colorectal cancer patients were enrolled in a single medical centre between 2003 and 2007. APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction. The primary outcome included 5-year overall survival and univariate (Kaplan-Meier) and multivariate (Cox regression) analyses of prognostic factors. Results: The results showed that 66 (33.8%) of 195 tumour tissues contained an APC mutation. The predominant APC gene variations were deletion mutations (50.0%). APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation. In advanced-stage cancer, patients with APC mutation/high miR-21 had poorer overall survival rates than those with APC mutation/low miR-21, APC wild-type/high miR-21 and APC wild-type/low miR-21. Conclusion: In Taiwan, downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation. APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC, especially in patients with advanced disease.

AB - Aim: Colorectal cancer (CRC) is the second commonest cause of cancer death in Taiwan. Although numerous genes have been associated with tumorigenesis in colorectal cancer, only a few have been validated and used as biomarkers for predicting clinical outcome. The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients. Method: In total, 195 colorectal cancer patients were enrolled in a single medical centre between 2003 and 2007. APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction. The primary outcome included 5-year overall survival and univariate (Kaplan-Meier) and multivariate (Cox regression) analyses of prognostic factors. Results: The results showed that 66 (33.8%) of 195 tumour tissues contained an APC mutation. The predominant APC gene variations were deletion mutations (50.0%). APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation. In advanced-stage cancer, patients with APC mutation/high miR-21 had poorer overall survival rates than those with APC mutation/low miR-21, APC wild-type/high miR-21 and APC wild-type/low miR-21. Conclusion: In Taiwan, downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation. APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC, especially in patients with advanced disease.

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