The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer

T. H. Chen, S. W. Chang, C. C. Huang, K. L. Wang, K. T. Yeh, C. N. Liu, H. Lee, C. C. Lin, Y. W. Cheng

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43 Citations (Scopus)

Abstract

Aim: Colorectal cancer (CRC) is the second commonest cause of cancer death in Taiwan. Although numerous genes have been associated with tumorigenesis in colorectal cancer, only a few have been validated and used as biomarkers for predicting clinical outcome. The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients. Method: In total, 195 colorectal cancer patients were enrolled in a single medical centre between 2003 and 2007. APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction. The primary outcome included 5-year overall survival and univariate (Kaplan-Meier) and multivariate (Cox regression) analyses of prognostic factors. Results: The results showed that 66 (33.8%) of 195 tumour tissues contained an APC mutation. The predominant APC gene variations were deletion mutations (50.0%). APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation. In advanced-stage cancer, patients with APC mutation/high miR-21 had poorer overall survival rates than those with APC mutation/low miR-21, APC wild-type/high miR-21 and APC wild-type/low miR-21. Conclusion: In Taiwan, downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation. APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC, especially in patients with advanced disease.

Original languageEnglish
Pages (from-to)1367-1374
Number of pages8
JournalColorectal Disease
Volume15
Issue number11
DOIs
Publication statusPublished - Nov 2013

Keywords

  • APC
  • MiR-21
  • Prognostic factor

ASJC Scopus subject areas

  • Gastroenterology

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    Chen, T. H., Chang, S. W., Huang, C. C., Wang, K. L., Yeh, K. T., Liu, C. N., Lee, H., Lin, C. C., & Cheng, Y. W. (2013). The prognostic significance of APC gene mutation and miR-21 expression in advanced-stage colorectal cancer. Colorectal Disease, 15(11), 1367-1374. https://doi.org/10.1111/codi.12318