The prognostic impact of RAP2A expression in patients with early and locoregionally advanced nasopharyngeal carcinoma in an endemic area

Ying En Lee, Hong Lin He, Tzu Ju Chen, Sung Wei Lee, I. Wei Chang, Chung Hsi Hsing, Chien Feng Li

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: By data mining from published transcriptomic databases, we identified RAP2A as a significantly upregulated gene in nasopharyngeal carcinoma (NPC) tissues. RAP2A, a member of the RAS oncogene family, is involved in the process of GTP binding and GTPase activity. The aim of this study was to evaluate the expression of RAP2A and its prognostic impact in patients with early and locoregionally advanced NPC. Methods: RAP2A immunohistochemistry was performed for 124 NPC patients who were receiving standard treatment and had no initial distal metastasis. We also performed Western blotting to evaluate the endogenous protein expression of RAP2A in NPC cells and non-neoplastic mucosal cells. The result of RAP2A expression was further correlated with clinicopathological variables, disease-specific survival (DSS), distant metastasis-free survival (DMeFS), and local recurrence-free survival (LRFS). Results: High expression of RAP2A was significantly associated with advanced primary tumor status (P = 0.024) and advanced TNM stage (P = 0.006). In univariate analysis, high expression of RAP2A served as a significant prognostic factor for inferior DSS (P <0.0001), DMeFS (P <0.0001), and LRFS (P <0.0001). In multivariate analysis, RAP2A overexpression still independently predicted worse DSS (hazard ratio [HR] = 2.976, P <0.001), DMeFS (HR = 4.233, P <0.001), and LRFS (HR = 4.156, P <0.001). Moreover, Both HONE1 and TW01 NPC cells, but not non-neoplastic DOK cells demonstrated significantly increased RAP2A expression. Conclusion: Overexpression of RAP2A is associated with advanced disease status and may therefore be an important prognosticator for poor outcomes in NPC, as well as a potential therapeutic target to aid in developing effective treatment modalities.

Original languageEnglish
Pages (from-to)912-921
Number of pages10
JournalAmerican Journal of Translational Research
Volume7
Issue number5
Publication statusPublished - Jul 7 2015
Externally publishedYes

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Hazards
Survival
Neoplasm Metastasis
GTP Phosphohydrolases
Guanosine Triphosphate
Data mining
Tumors
Recurrence
Genes
Tissue
Nasopharyngeal carcinoma
Data Mining
Oncogenes
Proteins
Therapeutics
Multivariate Analysis
Western Blotting
Immunohistochemistry
Databases
Neoplasms

Keywords

  • GTPase
  • Nasopharyngeal carcinoma
  • NPC
  • RAP2A

ASJC Scopus subject areas

  • Medicine(all)
  • Cancer Research
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

The prognostic impact of RAP2A expression in patients with early and locoregionally advanced nasopharyngeal carcinoma in an endemic area. / Lee, Ying En; He, Hong Lin; Chen, Tzu Ju; Lee, Sung Wei; Chang, I. Wei; Hsing, Chung Hsi; Li, Chien Feng.

In: American Journal of Translational Research, Vol. 7, No. 5, 07.07.2015, p. 912-921.

Research output: Contribution to journalArticle

Lee, Ying En ; He, Hong Lin ; Chen, Tzu Ju ; Lee, Sung Wei ; Chang, I. Wei ; Hsing, Chung Hsi ; Li, Chien Feng. / The prognostic impact of RAP2A expression in patients with early and locoregionally advanced nasopharyngeal carcinoma in an endemic area. In: American Journal of Translational Research. 2015 ; Vol. 7, No. 5. pp. 912-921.
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abstract = "Background: By data mining from published transcriptomic databases, we identified RAP2A as a significantly upregulated gene in nasopharyngeal carcinoma (NPC) tissues. RAP2A, a member of the RAS oncogene family, is involved in the process of GTP binding and GTPase activity. The aim of this study was to evaluate the expression of RAP2A and its prognostic impact in patients with early and locoregionally advanced NPC. Methods: RAP2A immunohistochemistry was performed for 124 NPC patients who were receiving standard treatment and had no initial distal metastasis. We also performed Western blotting to evaluate the endogenous protein expression of RAP2A in NPC cells and non-neoplastic mucosal cells. The result of RAP2A expression was further correlated with clinicopathological variables, disease-specific survival (DSS), distant metastasis-free survival (DMeFS), and local recurrence-free survival (LRFS). Results: High expression of RAP2A was significantly associated with advanced primary tumor status (P = 0.024) and advanced TNM stage (P = 0.006). In univariate analysis, high expression of RAP2A served as a significant prognostic factor for inferior DSS (P <0.0001), DMeFS (P <0.0001), and LRFS (P <0.0001). In multivariate analysis, RAP2A overexpression still independently predicted worse DSS (hazard ratio [HR] = 2.976, P <0.001), DMeFS (HR = 4.233, P <0.001), and LRFS (HR = 4.156, P <0.001). Moreover, Both HONE1 and TW01 NPC cells, but not non-neoplastic DOK cells demonstrated significantly increased RAP2A expression. Conclusion: Overexpression of RAP2A is associated with advanced disease status and may therefore be an important prognosticator for poor outcomes in NPC, as well as a potential therapeutic target to aid in developing effective treatment modalities.",
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T1 - The prognostic impact of RAP2A expression in patients with early and locoregionally advanced nasopharyngeal carcinoma in an endemic area

AU - Lee, Ying En

AU - He, Hong Lin

AU - Chen, Tzu Ju

AU - Lee, Sung Wei

AU - Chang, I. Wei

AU - Hsing, Chung Hsi

AU - Li, Chien Feng

PY - 2015/7/7

Y1 - 2015/7/7

N2 - Background: By data mining from published transcriptomic databases, we identified RAP2A as a significantly upregulated gene in nasopharyngeal carcinoma (NPC) tissues. RAP2A, a member of the RAS oncogene family, is involved in the process of GTP binding and GTPase activity. The aim of this study was to evaluate the expression of RAP2A and its prognostic impact in patients with early and locoregionally advanced NPC. Methods: RAP2A immunohistochemistry was performed for 124 NPC patients who were receiving standard treatment and had no initial distal metastasis. We also performed Western blotting to evaluate the endogenous protein expression of RAP2A in NPC cells and non-neoplastic mucosal cells. The result of RAP2A expression was further correlated with clinicopathological variables, disease-specific survival (DSS), distant metastasis-free survival (DMeFS), and local recurrence-free survival (LRFS). Results: High expression of RAP2A was significantly associated with advanced primary tumor status (P = 0.024) and advanced TNM stage (P = 0.006). In univariate analysis, high expression of RAP2A served as a significant prognostic factor for inferior DSS (P <0.0001), DMeFS (P <0.0001), and LRFS (P <0.0001). In multivariate analysis, RAP2A overexpression still independently predicted worse DSS (hazard ratio [HR] = 2.976, P <0.001), DMeFS (HR = 4.233, P <0.001), and LRFS (HR = 4.156, P <0.001). Moreover, Both HONE1 and TW01 NPC cells, but not non-neoplastic DOK cells demonstrated significantly increased RAP2A expression. Conclusion: Overexpression of RAP2A is associated with advanced disease status and may therefore be an important prognosticator for poor outcomes in NPC, as well as a potential therapeutic target to aid in developing effective treatment modalities.

AB - Background: By data mining from published transcriptomic databases, we identified RAP2A as a significantly upregulated gene in nasopharyngeal carcinoma (NPC) tissues. RAP2A, a member of the RAS oncogene family, is involved in the process of GTP binding and GTPase activity. The aim of this study was to evaluate the expression of RAP2A and its prognostic impact in patients with early and locoregionally advanced NPC. Methods: RAP2A immunohistochemistry was performed for 124 NPC patients who were receiving standard treatment and had no initial distal metastasis. We also performed Western blotting to evaluate the endogenous protein expression of RAP2A in NPC cells and non-neoplastic mucosal cells. The result of RAP2A expression was further correlated with clinicopathological variables, disease-specific survival (DSS), distant metastasis-free survival (DMeFS), and local recurrence-free survival (LRFS). Results: High expression of RAP2A was significantly associated with advanced primary tumor status (P = 0.024) and advanced TNM stage (P = 0.006). In univariate analysis, high expression of RAP2A served as a significant prognostic factor for inferior DSS (P <0.0001), DMeFS (P <0.0001), and LRFS (P <0.0001). In multivariate analysis, RAP2A overexpression still independently predicted worse DSS (hazard ratio [HR] = 2.976, P <0.001), DMeFS (HR = 4.233, P <0.001), and LRFS (HR = 4.156, P <0.001). Moreover, Both HONE1 and TW01 NPC cells, but not non-neoplastic DOK cells demonstrated significantly increased RAP2A expression. Conclusion: Overexpression of RAP2A is associated with advanced disease status and may therefore be an important prognosticator for poor outcomes in NPC, as well as a potential therapeutic target to aid in developing effective treatment modalities.

KW - GTPase

KW - Nasopharyngeal carcinoma

KW - NPC

KW - RAP2A

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