類胡蘿蔔素對檳榔嚼塊萃取物誘發倉鼠口腔癌症之預防效果

Translated title of the contribution: The preventive effect of carotenoids on betel quid extract induced oral carcinoma in hamster

H. Chien, S. Y. Huang, Y. H. Lin, Y. H. Shieh, Ming-Che Hsieh, H. F. Wang, M. J. Shieh

Research output: Contribution to journalArticle

Abstract

The incidence of oral cancer is highly associated with betel quid chewing in Taiwan. Previous studies have shown that betel quid represents a promoter role during the carcinogenesis of hamster buccal pouch carcinoma. The proposed study investigated the preventive effects of carotenoids on betel quid extract (BQE) induced hamster oral cancer. Forty hamsters were divided into six experimental groups and treated with 0.3 mL 0.5% experimental agents, which including DMBA (9, 10-dimethyl-1, 2-benz[a]anthracene), BQE, α-tocopherol, β-carotene, lycopene, lutein and a mixture of equal amounts of carotenoid and vitamin E. In 16-week experimental period, tested groups were initially daubed with DMBA for 4 weeks. In the following 12 weeks, groups were daubed with either BQE or a mineral oil antioxidants solution three times per week on alternative days. At the end of the experiment, the pouch and esophagus of each group was sampled, routinely processed for paraffin sections, and evaluated by a senior pathologist. The results indicated buccal or esophageal histological symptoms in the mixture group were less severe compared with the controlled group. By DMBA induction, the BQE intervention indeed promoted the development of oral cancer. Carotenoids present effectively inhibited the development of oral mucosal malignant tumors, especially in the lycopene and the mixture group. Compared with the esophageal and buccal mucosal pathology, the number of buccal mucosa tumors was less than those in the esophagus. However, the volume of tumors in the esophagus was smaller than the buccal tumors. Furthermore, the buccal mucosa tumor burden was much greater than those in the esophagus. From the carotenoid preventive viewpoint, the tumor number and burden in the esophagus and the buccal mucosa were the most severe in the controlled group. The application of carotenoids could significantly inhibit the development of both esophageal and buccal tumors, especially in the mixture group. In summary, the four different carotenoids significantly inhibited the incidence of oral cancer when administered alone as well as with a mixture of antioxidants, which produced a significant synergistic oral cancer chemoprevention.

Translated title of the contributionThe preventive effect of carotenoids on betel quid extract induced oral carcinoma in hamster
Original languageTraditional Chinese
Pages (from-to)98-108
Number of pages11
JournalNutritional Sciences Journal
Volume26
Issue number2
Publication statusPublished - 2001

Keywords

  • α-tocopherol
  • β-carotene
  • Carotenoids
  • Lutein
  • Lycopene
  • Oral cancer

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

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