The predictive role of E2-EPF ubiquitin carrier protein in esophageal squamous cell carcinoma

Miao Fen Chen, Kuan Der Lee, Ming Shian Lu, Chih Cheng Chen, Ming Ju Hsieh, Yun Hen Liu, Paul Yang Lin, Wen Cheng Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The ubiquitin proteasome pathway has been implicated in carcinogenesis. However, the role of E2-EPF ubiquitin carrier protein (UCP) in esophageal cancer remains relatively unstudied. In the study, we examined the mRNA level of circulating tumor cells from 60 esophageal cancer patients by membrane arrays consisting of a panel of potential markers including UCP, compared to 40 normal populations. The predictive capacity of UCP was also assessed by immunohistochemical staining of a retrospective series of 84 biopsied esophageal squamous cell carcinomas in relation to clinical outcome. In addition, we studied in vitro biological changes including tumor growth, metastatic capacity, and the sensitivity to irradiation and cisplatin, after experimental manipulation of UCP expression in esophageal cancer cells. By the data of 25-gene membrane array analysis, UCP was the only factor significantly associated with the extent of tumor burden in esophageal cancer patients. Our immunochemistry findings further indicate that UCP positivity was linked to poor response to neoadjuvant therapy and worse survival. In cell culture, inhibited UCP significantly decrease tumor growth and the capacity for metastasis. The epithelial-mesenchymal transition (EMT) induced by VHL/HIF-1α-TGF-β1 pathway might be the underlying mechanism responsible to the more aggressive tumor growth in UCP-positive esophageal cancer. Our results suggest that UCP was significantly associated with poor prognosis of esophageal cancer and may be a new molecular target for therapeutic intervention for esophageal squamous cell carcinoma.

Original languageEnglish
Pages (from-to)307-320
Number of pages14
JournalJournal of Molecular Medicine
Volume87
Issue number3
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Esophageal Neoplasms
Growth
Circulating Neoplastic Cells
Immunochemistry
ubiquitin carrier proteins
Esophageal Squamous Cell Carcinoma
Neoplasms
Neoadjuvant Therapy
Epithelial-Mesenchymal Transition
Membranes
Proteasome Endopeptidase Complex
Ubiquitin
Tumor Burden
Cisplatin
Carcinogenesis
Cell Culture Techniques
Staining and Labeling
Neoplasm Metastasis
Messenger RNA
Survival

Keywords

  • E2-EPF ubiquitin carrier protein (UCP)
  • Epithelial mesenchymal transition
  • Esophageal cancer
  • Membrane array
  • Response to neoadjuvant CCRT

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery
  • Genetics(clinical)

Cite this

The predictive role of E2-EPF ubiquitin carrier protein in esophageal squamous cell carcinoma. / Chen, Miao Fen; Lee, Kuan Der; Lu, Ming Shian; Chen, Chih Cheng; Hsieh, Ming Ju; Liu, Yun Hen; Lin, Paul Yang; Chen, Wen Cheng.

In: Journal of Molecular Medicine, Vol. 87, No. 3, 03.2009, p. 307-320.

Research output: Contribution to journalArticle

Chen, Miao Fen ; Lee, Kuan Der ; Lu, Ming Shian ; Chen, Chih Cheng ; Hsieh, Ming Ju ; Liu, Yun Hen ; Lin, Paul Yang ; Chen, Wen Cheng. / The predictive role of E2-EPF ubiquitin carrier protein in esophageal squamous cell carcinoma. In: Journal of Molecular Medicine. 2009 ; Vol. 87, No. 3. pp. 307-320.
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