Abstract

Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.

Original languageEnglish
Article number250615
JournalJournal of Nanomaterials
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Cartilage
Platelets
Collagen
Scaffolds
Scaffolds (biology)
Plasmas
Aggrecans
Collagen Type II
Tissue engineering
Cells
Messenger RNA
Immunohistochemistry

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

The potential use of platelet-rich plasma to reconstruct the microtia chondrocyte in human auricular cartilage regeneration. / Chen, Wei Hong; Liu, Hen Yu; Tsai, Ching Yu; Wu, Chia Che; Wei, Hong Jian; Liu, Alice; Lai, Ming-Tang; Huang, Chiung Fang; Deng, Win Ping.

In: Journal of Nanomaterials, Vol. 2015, 250615, 2015.

Research output: Contribution to journalArticle

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abstract = "Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.",
author = "Chen, {Wei Hong} and Liu, {Hen Yu} and Tsai, {Ching Yu} and Wu, {Chia Che} and Wei, {Hong Jian} and Alice Liu and Ming-Tang Lai and Huang, {Chiung Fang} and Deng, {Win Ping}",
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AU - Chen, Wei Hong

AU - Liu, Hen Yu

AU - Tsai, Ching Yu

AU - Wu, Chia Che

AU - Wei, Hong Jian

AU - Liu, Alice

AU - Lai, Ming-Tang

AU - Huang, Chiung Fang

AU - Deng, Win Ping

PY - 2015

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AB - Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.

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