Abstract

We examined a possible role for heat shock factor-1 (HSF-1) in the negative regulation of HO-1 gene expression in human Hep3B hepatoma cells responding to stimulation with 15-deoxy-Δ 12,14-prostaglandin J 2 (15d-PGJ 2) and arsenite. Overexpression of HSF-1 and heat-shock experiments indicated that HSF-1 repressed the 15d-PGJ 2-and arsenite-induced HO-1 gene expression through directly binding to the consensus heat shock element (HSE) of the HO-1 gene promoter. In addition, point mutations at specific HSE sequences of the HO-1 promoter-driven luciferase plasmid (pGL2/hHO3.2-Luc) abolished the heat shock- and HSF-1-mediated repression of reporter activity. Overall, it is possible that HSF-1 negatively regulates HO-1 gene expression, and that the HSE present in the -389 to -362 region mediates HSF-1-induced repression of human HO-1 gene expression.

Original languageEnglish
Pages (from-to)604-615
Number of pages12
JournalInternational Journal of Biochemistry and Cell Biology
Volume37
Issue number3
DOIs
Publication statusPublished - Mar 2005

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Keywords

  • 15-Deoxy- Δ -prostaglandin J
  • Arsenite
  • Heat shock factor-1
  • Heme oxygenase-1
  • Hepatoma

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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