The positional capture experiments of the LCM using near field fiber probe

Chi Fu Yen, Jen Ai Lee, Chien Ming Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser Capture Microdissection (LCM) is a technique that permits rapid and reliable procurement of pure population of cells from tissue sections. We created the LCM system with a near field fiber probe to transmit the laser light (808 nm) to heat the thermoplastic polymer film which was placed above the tissue section. Laser spots in nano-dimension cause the film to be melted and fused with the underlying target of choice. In the study, we set the fiber probe on a two-dimensional nanometer PZT to do precisely capture. The aperture of fiber probe we used is 100 nm through which light can be focused. We used 20 nm spread gold particles to be captured in these experiments instead of tissue sections. By moving the probe in X direction to melt spots in different distances, we can know more molecular properties of the thermoplastic polymer film especially in its resolving power. When the film is removed, the chosen particles remain bound to the film, while the rest of the tissue or particles are left behind. According to the results of this study, we can know how close the melted spots can reach to, and it is also helpful to the operation of this new LCM system.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsC. Quan, F.S. Chau, A. Asundi, B.S. Wong, C.T. Lim
Pages844-848
Number of pages5
Volume5852 PART II
DOIs
Publication statusPublished - 2005
EventThird International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics - Singapore, Singapore
Duration: Nov 29 2004Dec 1 2004

Other

OtherThird International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics
CountrySingapore
CitySingapore
Period11/29/0412/1/04

Fingerprint

Microdissection
near fields
fibers
Fibers
Lasers
probes
Tissue
lasers
Polymer films
Experiments
Thermoplastics
procurement
molecular properties
Optical resolving power
polymers
Gold
apertures
Cells
gold
heat

Keywords

  • Laser capture microdissection
  • Near filed
  • Probe

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Yen, C. F., Lee, J. A., & Chen, C. M. (2005). The positional capture experiments of the LCM using near field fiber probe. In C. Quan, F. S. Chau, A. Asundi, B. S. Wong, & C. T. Lim (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5852 PART II, pp. 844-848). [133] https://doi.org/10.1117/12.621916

The positional capture experiments of the LCM using near field fiber probe. / Yen, Chi Fu; Lee, Jen Ai; Chen, Chien Ming.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / C. Quan; F.S. Chau; A. Asundi; B.S. Wong; C.T. Lim. Vol. 5852 PART II 2005. p. 844-848 133.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yen, CF, Lee, JA & Chen, CM 2005, The positional capture experiments of the LCM using near field fiber probe. in C Quan, FS Chau, A Asundi, BS Wong & CT Lim (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5852 PART II, 133, pp. 844-848, Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, Singapore, Singapore, 11/29/04. https://doi.org/10.1117/12.621916
Yen CF, Lee JA, Chen CM. The positional capture experiments of the LCM using near field fiber probe. In Quan C, Chau FS, Asundi A, Wong BS, Lim CT, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5852 PART II. 2005. p. 844-848. 133 https://doi.org/10.1117/12.621916
Yen, Chi Fu ; Lee, Jen Ai ; Chen, Chien Ming. / The positional capture experiments of the LCM using near field fiber probe. Proceedings of SPIE - The International Society for Optical Engineering. editor / C. Quan ; F.S. Chau ; A. Asundi ; B.S. Wong ; C.T. Lim. Vol. 5852 PART II 2005. pp. 844-848
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