The phenolic constituent and free radical scavenging activities of Gynura formosona Kiamnra

Wen Chi Hou, Rong Dih Lin, Zong-Huei Li, Ying Hua Huang, Feng-Lin Hsu, Mei Hsien Lee

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Gynura formosana Kiamnra (Compositae) is a herbal folk medicine that is a popular vegetable in Taiwan. The free-radical scavenging activities of a 70% aqueous acetone extract from the herb G formosana were evaluated. Bioassay-guided fractionation, column separation on Diaion, Toyopearl HW 40(C), Sephadex LH-20 and MCI CHP20P, and high-performance liquid chromatography (HPLC) were used to isolate for the first time in G formosana four potent phenolics [caffeic acid (I), quercetin 3-O-rutinoside (II), kaempferol 3-O-rutinoside (III) and kaempferol 3-O-robinobioside (IV)]. The IC50 values of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity for compounds I-IV were 6.7, 7.7, 300.3 and 286.7 μM, respectively, and, for superoxide radical scavenging activity, they were 187.3, 25.8, 55.3 and 87.4 μM, respectively. Using a spin trapping electron spin resonance (ESR) method, caffeic acid (I) and quercetin 3-O-rutinoside (II) exhibited good hydroxyl radical activity. The free radical scavenging activity of G formosana phenolics may improve the economic value of this herb and assist in its development as a health food.

Original languageEnglish
Pages (from-to)615-621
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume85
Issue number4
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Gynura
Scavenging
rutin
Free Radicals
kaempferol
caffeic acid
Spin Trapping
quercetin
Asteraceae
Herbal Medicine
herbs
Electron Spin Resonance Spectroscopy
Traditional Medicine
Acetone
Taiwan
Superoxides
Biological Assay
Vegetables
Hydroxyl Radical
Inhibitory Concentration 50

Keywords

  • Caffeic acid
  • Free-radical scavenging
  • Gynura formosana
  • Kaempferol 3-O-robinobioside
  • Kaempferol 3-O-rutinoside
  • Quercetin 3-O-rutinoside

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

The phenolic constituent and free radical scavenging activities of Gynura formosona Kiamnra. / Hou, Wen Chi; Lin, Rong Dih; Li, Zong-Huei; Huang, Ying Hua; Hsu, Feng-Lin; Lee, Mei Hsien.

In: Journal of the Science of Food and Agriculture, Vol. 85, No. 4, 03.2005, p. 615-621.

Research output: Contribution to journalArticle

Hou, Wen Chi ; Lin, Rong Dih ; Li, Zong-Huei ; Huang, Ying Hua ; Hsu, Feng-Lin ; Lee, Mei Hsien. / The phenolic constituent and free radical scavenging activities of Gynura formosona Kiamnra. In: Journal of the Science of Food and Agriculture. 2005 ; Vol. 85, No. 4. pp. 615-621.
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