The novel isoflavone derivatives inhibit RANKL-induced osteoclast formation

Chih Hsin Tang, Chih Shiang Chang, Tzu Wei Tan, Shan Chi Liu, Ju Fang Liu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Isoflavones are compounds structurally similar to the mammalian estrogens and have received considerable attention for their preventive actions on bone loss. Here, we synthesized the novel isoflavone derivatives and examined their activities in bone cells. We found that the novel isoflavone derivatives markedly inhibited the receptor activator of nuclear factor kappa B ligand (RANKL) plus macrophage colony stimulating factor (M-CSF)-induced osteoclastic differentiation from bone marrow stromal cells and RAW264.7 macrophage cells. Treatment of RAW264.7 macrophages with RANKL-induced extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) phosphorylation. However, RANKL-induced p38 and JNK but not ERK phosphorylation was attenuated by isoflavone derivatives. Furthermore, RANKL-mediated increase of p65 phosphorylation at Ser536, NF-κB-specific DNA-protein complex formation and κB-luciferase activity was inhibited by isoflavone derivatives. On the other hand, isoflavone derivatives did not affect the cell proliferation and differentiation of human cultured osteoblasts. Our data suggest that the novel isoflavone derivatives inhibit osteoclastogenesis from bone marrow stromal cells and macrophage cells via attenuation of RANKL-induced p38, JNK and NF-κB activation.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalEuropean Journal of Pharmacology
Volume648
Issue number1-3
DOIs
Publication statusPublished - Dec 1 2010

Keywords

  • Chinese herb
  • Isoflavones
  • Osteoclast
  • Osteoporosis
  • RANKL

ASJC Scopus subject areas

  • Pharmacology

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