The novel herbal cocktail aga alleviates oral cancer through inducing apoptosis, inhibited migration and promotion of cell cycle arrest at subg1 phase

Jui Hua Lu, Yen Ru Chou, Yue Hua Deng, Mao Suan Huang, Shaw Ting Chien, Bach Thi Nhu Quynh, Chia Yu Wu, Edlin Anahi Peláez Achtmann, Hsin Chung Cheng, Navneet Kumar Dubey, Win Ping Deng

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Traditional Chinese medicines Antler’s extract (A) and Ganoderma lucidum (G) and Antrodia Camphorata (A) have been known to individually contain a plethora of bioactive factors including triterpenoids, polysaccharides etc., exerting various curative impacts such as anti-inflammatory, anti-oxidative, anti-atherosclerotic and anti-viral activities. However, their combinatorial therapeutic efficacy for oral cancer has not been investigated. Hence, we synthesized a robust cocktail called AGA and investigated its anti-oral cancer potential in vitro and in vivo. An MTT assay revealed the IC50 of AGA to be about 15 mg at 72 h. Therefore, 10 mg and 20 mg doses were selected to study the effect of AGA. The AGA significantly inhibited proliferation of oral cancer cells (HSC3, SAS, and OECM-1) in a dose-and time-dependent manner. AGA retarded cell cycle regulators (CDK4, CDK6, cyclin A, B1, D1 and E2) and apoptosis inhibitory protein Bcl-2, but enhanced pro-apoptotic protein Bax and a higher percentage of cells in Sub-G1 phase. Mechanistically, AGA suppressed all EMT markers; consequently, it decreased the migration ability of cancer cells. AGA significantly reduced xenograft tumor growth in nude mice with no adverse events in liver and renal toxicity. Conclusively, AGA strongly inhibited oral cancer through inducing apoptosis and inhibiting the migration and promotion of cell cycle arrest at subG1 phase, which may be mediated primarily via cocktail-contained triterpenoids and polysaccharides.

Original languageEnglish
Article number3214
Pages (from-to)1-15
Number of pages15
JournalCancers
Volume12
Issue number11
DOIs
Publication statusPublished - Nov 2020

Keywords

  • Anti-oxidation
  • Antler’s extract
  • Antrodia Camphorata
  • Cell cycle
  • Ganoderma lucidum
  • Oral cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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