The mucilage of yam (Dioscorea batatas Decne) tuber exhibited angiotensin converting enzyme inhibitory activities

Mei Hsien Lee, Yin Shiou Lin, Yaw Huei Lin, Feng-Lin Hsu, Wen Chi Hou

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The tuber mucilage of yam (Dioscorea batatas Decne) (YTM) was extracted and purified to homogeneity, which was confirmed by the toluidine blue staining on a sodium dodecylsulfate-polyacrylamide gel electrophoresis gel treated with 2-mercaptoethanol appearing as a single band with molecular mass larger than 250 kDa. This purified YTM was shown by spectrophotometric method to inhibit angiotensin converting enzyme (ACE) in a dose-dependent manner (28.7 to 59.8% ACE inhibition, respectively, by 102.46 to 409.84 μg/mL YTM) using (N-(3-[2-furyl] acryloyl)-Phe-Gly-Gly) (FAPGG) as a substrate. The concentration of YTM required for 50% inhibition (IC50) of ACE activity was 256.2 μg/mL while that of captopril was 0.00781 μM (0.0095 nmole). The commercial polysaccharide pectin (102.46 to 307.38 μg/mL) showed no inhibitory activity against ACE. Using fluorescent silica TLC or C 18 reverse phase HPLC to detect FAPGG and FAP, the results also showed that YTM inhibited ACE. The YTM showed mixed type inhibition against ACE, and the Michaelis constant in the presence of YTM was 0.33 mM. Consumption of yam tubers may benefit people's health.

Original languageEnglish
Pages (from-to)267-273
Number of pages7
JournalBotanical Bulletin of Academia Sinica
Volume44
Issue number4
Publication statusPublished - Oct 2003

Fingerprint

Dioscorea polystachya
mucilages
yams
peptidyl-dipeptidase A
tubers
toluidine blue
enzyme inhibition
silica
pectins
polyacrylamide gel electrophoresis
inhibitory concentration 50
polysaccharides
sodium
gels
enzyme activity
molecular weight
dosage

Keywords

  • Angiotensin converting enzyme (ACE)
  • HPLC
  • Mucilage
  • N-(3-[2-furyl]acryloyl-Phe-Gly- Gly) (FAPGG)
  • TLC
  • Yam

ASJC Scopus subject areas

  • Plant Science

Cite this

The mucilage of yam (Dioscorea batatas Decne) tuber exhibited angiotensin converting enzyme inhibitory activities. / Lee, Mei Hsien; Lin, Yin Shiou; Lin, Yaw Huei; Hsu, Feng-Lin; Hou, Wen Chi.

In: Botanical Bulletin of Academia Sinica, Vol. 44, No. 4, 10.2003, p. 267-273.

Research output: Contribution to journalArticle

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